壬基酚和辛基酚联合暴露的血清代谢特征及生物标志物  

Serum metabolism characteristics and biomarker of joint exposure to nonylphenol and octylphenol

作　　者：柳春红[1,2] 王伟华[1] 孙远明[1,2] 林峰[3] 黄少文[1] LIU Chun-hong;WANG Wei-hua;SUN Yuan-ming;LIN Feng;HUANG Shao-wen(Food College, South China Agricultural University, Guangzhou 510642, China;Guangdong Provincial Key Laboratory of Food Quality and Safety,Guangzhou 510642, China;Guangdong Inspection and Quarantine Technology Center Food Testing Lab, Guangzhou 510623, China)

机构地区：[1]华南农业大学食品学院,广东广州510642 [2]广东省食品质量安全重点实验室,广东广州510642 [3]广东出入境检验检疫局检验检疫技术中心食品实验室,广东广州510623

出　　处：《中国环境科学》2016年第11期3502-3507,共6页China Environmental Science

基　　金：农业部农产品质量安全风险评估专项(GJFP201501202);广东省自然科学基金项目(2016A030313395);广东省科技计划项目(2013B020204001)

摘　　要：运用代谢组学方法分析了壬基酚(NP)和辛基酚(OP)联合暴露后大鼠血清代谢成分变化,雄性SD大鼠随机分为对照组、壬基酚和辛基酚(联合染毒低剂量组(25mg/kg NP+25mg/kg OP)及高剂量组(75mg/kg NP+75mg/kg OP).连续灌胃7d后处死.通过超高效液相色谱-飞行时间质谱(UPLC/QTOF-MS)技术获得大鼠血清的代谢指纹图谱,通过主成分分析观察了联合染毒的剂量-效应,通过t检验筛选潜在的生物标志物,并结合代谢物数据库检索对潜在的生物标志物进行鉴定.结果显示,对照组、高、低剂量组各组间代谢谱有明显差别,且随着染毒剂量的增加毒性增强,表现为剂量-效应关系.联合染毒后的血清代谢物中,含量升高的有牛黄胆酸及1-棕榈酰基磷脂酰胆碱,含量降低的有酪氨酸、5-尿苷一磷酸及硫脑苷酯,提示染毒可能对内分泌系统、心血管系统、神经系统以及核苷酸合成、糖代谢、磷脂代谢等产生不良影响.The metabonomics method was employed to investigate the changes in serum metabolic components ofSprague Dawley rats after combined exposure to nonylphenol (NP) and octylphenol (OP). The male rats were randomlydivided into control group, low-dose combined exposure group (25mg/kg NP + 25mg/kg OP) and high-dose combinedexposure group (75mg/kg NP + 75mg/kg OP). The rats were treated by gavage once a day for 7 days, and then killed. Themetabolic fingerprinting of rat serum was obtained by ultra-performance liquid chromatography coupled withtime-of-flight mass spectrometry (UPLC/TOF-MS). Dose-response toxicity was observed based on the principlecomponents analysis (PCA). Meanwhile, the potential biomarkers were screened out according to t-test. The resultsshowed that there was a significant difference in the metabolic profile among the control group, high and low dose groups,and with the increase of exposure dose, the toxicity was enhanced, which showed a dose-effect relationship. Meanwhile,the contents of taurocholic acid and 1-Palmitoyllysophosphatidylcholine increased while the contents of tyrosine,uridine-5′- monophosphate and sulfatide decreased under combined exposure to NP and OP. It is suggested that NP andOP exposure may have adverse effects on the endocrine system, cardiovascular system, nervous system, nucleotidesynthesis, sugar and phospholipid metabolism.

关 键 词：壬基酚 辛基酚 联合暴露 代谢特征 生物标志物 

分 类 号：X503.22[环境科学与工程—环境工程]