SISH在乳腺癌HER-2基因检测中的应用价值  

The value of silver-enhanced in situ hybridization technology in HER-2 detection of breast carcinoma

作　　者：周冬梅[1] 陈刚[1] 师怡[1] 何银珠[1] 郑雄伟[1] Zhou Dongmei;Chen Gang;Shi Yi;He Yinzhu;Zheng Xiongwei(Department o f Pathology, Fujian Cancer Hospital’Affiliated Hospital o f Fujian Medical University, Key Laboratory o f Carcinogenesis and Translational Research^ Fujian Province), Fujian Fuzhou 350014,China)

机构地区：[1]福建医科大学附属医院,福建省肿瘤医院病理科,福建省肿瘤转化医学重点实验室,福建福州350014

出　　处：《现代肿瘤医学》2016年第14期2247-2250,共4页Journal of Modern Oncology

基　　金：福建省卫生系统中青年骨干人才培养项目(编号:2015-ZQN-JC-8);国家临床重点专科建设项目(2013)

摘　　要：目的:探讨全自动银染原位杂交(SISH)方法在乳腺浸润性导管癌组织HER-2基因检测中的应用价值。方法:分别采用免疫组化(IHC)、荧光原位杂交(FISH)、银染原位杂交(SISH)方法对105例乳腺浸润性导管癌组织中的HER-2基因进行检测。结果:HER-2的IHC检测(-)、(1+)、(2+)、(3+)者与FISH检测扩增的符合率分为100%、12.5%、46.5%、89.5%;SISH和FISH检测结果的符合率为100%。结论:SISH是一种快速、准确且重复性高的HER-2基因检测方法,可作为FISH的替代技术。Objective: To evaluate HER - 2 gene expression of invasive breast carcinoma tissues determined bysilver enhanced in situ hybridization(SISH) technique. Methods:Immunohistochemistry (IHC) and fluorescence insitu hybridization( FISH) and SISH was used for HER - 2 gene detection of 105 cases. Results: The coincidencerates of IHC ( - ),(l+),(2 + ),(3 + ) with FISH detection about HER - 2 gene were 100% , 12. 5% ,46. 9% ,89. 5% Respectively. The coincidence rate of SISH with FISH about HER -2 gene amplification were 100%. Conclu-sion: SISH is a rapid, accurate and reproducible detection methods for HER -2 gene. It is a potenial alternative meth-od of FISH.

关 键 词：乳腺浸润性导管癌 HER-2 免疫组化 荧光原位杂交 银染色法 

分 类 号：R737.9[医药卫生—肿瘤]