PRC1高表达与前列腺癌生化复发的相关性研究  

Correlation analysis between overexpression of protein regulator of cytokinesis 1 and biochemical recurrence of prostate cancer

作　　者：韩兆冬[1,2] 罗宏伟 林卓远 梁应科 陈果[2] 吴永定 何慧婵[3] Han Zhaodong;Luo Hongwei;Lin Zhuoyuan;Liang Yingke;Chen Guo;Wu Yongding;He Huichan(Department o f Urology, Guangzhou First People’s Hospital, Guangzhou Medical University,Guangzhou 510180, China)

机构地区：[1]广州市第一人民医院泌尿外科,广州510180 [2]广州市第一人民医院中心实验室,广州510180 [3]广州医科大学附属第一医院,广东省泌尿外科重点实验室,广州510230

出　　处：《新医学》2016年第7期442-446,共5页Journal of New Medicine

基　　金：广东省自然科学基金(2014A030310066);广州市卫生局一般引导项目(20141A010013);广东省科技计划项目(2013B021800055);广州市科技计划项目(2014J4100072)

摘　　要：目的研究细胞质分裂调控蛋白1(PRC1)在前列腺癌中的表达情况及其与生化复发的相关性。方法采用实时定量PCR及蛋白免疫印迹法检测PRC1在前列腺癌与癌旁前列腺组织的表达水平,并利用Taylor基因芯片数据库对PRC1基因mRNA水平进行验证。Kaplan-Meier法分析前列腺癌患者总体生存率及生化复发时间与PRC1 mRNA表达水平之间的关系。Cox回归分析临床病理特征与生化复发的相关性。结果 12对前列腺癌及癌旁前列腺组织中,前列腺癌的PRC1 mRNA及蛋白表达均高于癌旁前列腺组织(P均<0.01)。Taylor基因芯片数据库分析显示,PRC1在前列腺癌组织中的表达水平高于非癌组织(P<0.001),且出现生化复发或转移、Gleason评分高、临床分期≥T3A期者的PRC1 mRNA表达水平高于无出现生化复化或转移、Gleason评分低、临床分期<T3A期者(P均<0.05);Kaplan-Meier分析显示,PRC1高表达组的无生化复发率明显低于PRC1低表达组(P=0.008);Cox回归分析显示,PRC1(P=0.001)、Gleason评分(P<0.001)、术前PSA水平(P=0.021)及病理分期(P=0.021)是前列腺癌生化复发的影响因素。结论 PRC1在前列腺癌中的高表达与生化复发呈正相关,PRC1表达水平有助于判断前列腺癌患者的预后。Objective To investigate the correlation between the expression level of protein regulatorof cytokinesis 1 ( PRC1) and biochemical recurrence of prostate cancer. Methods The expression levels ofPRC1 protein and mRNA between the prostate cancer and adjacent benign tissues were statistically compared usingWestern blot and RT-PCR. The expression of PRC1 mRNA was validated by microarray-based Taylor database.Kaplan-Meier plot was performed to evaluate the overall survival and analyze the correlation betweenPRC1 expression and biochemical recurrence of prostate cancer. Cox proportional hazards regression model wasutilized to assess the correlation between clinicopathological characteristics and biochemical recurrence of prostatecancer. Results Among 12 pairs of prostate cancer and adjacent benign tissues, the expression levels ofPRC1 protein and mRNA in the prostate cancer tissues were significantly higher than those in the adjacent benignprostate tissues (both P <0. 01). Microarray-based Taylor database revealed that the expression of PRC1in the cancer tissues was significantly up-regulated compared with that in the non-cancerous tissues ( P <0. 001). In addition, the expression of PRC1 mRNA in patients with biochemical recurrence or metastasis ofprostate cancer, high Gleason score and clinical staging ^T 3A was significantly up-regulated compared withtheir counterparts with no biochemical recurrence or metastasis of prostate cancer, low Gleason score and clinicalstaging < T3A (all P < 0. 05 ). Kaplan-Meier plot demonstrated that the biochemical recurrence rate ofprostate cancer in patients with PRC1 overexpression was considerably lower than that in those with low PRC1expression (P = 0. 008). Cox proportional hazards regression model revealed that PRC1 (P = 0. 001) , Gleasonscore ( P <0. 001) , preoperative prostate specific antigen level (P = 0. 021) and pathological staging ( P=0. 021) were associated with the biochemical recurrence of prostate cancer. Conclusions Overexpression ofPRC1 is positively correlated wit

关 键 词：细胞质分裂调控蛋白1 前列腺癌 生化复发 预后 

分 类 号：R737.25[医药卫生—肿瘤]