桑色素对全氟辛酸诱导小鼠肝损伤的保护作用  

Protective Effect of Morin on Perfluorooctanoic Acid-Induced Liver Injury in Mice

作　　者：余舒文 陶恩威 苑洋洋[2] 刘稳稳 吴娅玲[2] 胡炜华[3] 杨蓓[2] 吴磊[2] 张大雷[2] YU Shu-wen;TAO En-wei;YUAN Yang-yang;LIU Wen-wen;WU Ya-ling;HU Wei-hua;WU Lei;ZHANG Da-lei;YANG Bei

机构地区：[1]南昌大学第一临床医学院,2012级南昌330006 [2]南昌大学基础医学院生理学教研室,南昌330006 [3]南昌大学期刊社,南昌330006

出　　处：《南昌大学学报（医学版）》2016年第3期6-9,107,共4页Journal of Nanchang University：Medical Sciences

基　　金：国家自然科学基金(81560248,81060056)

摘　　要：目的探讨桑色素(morin,Mor)对全氟辛酸(perfluorooctanoic acid,PFOA)诱导小鼠肝损伤的保护作用。方法将24只雄性小鼠按随机数字表法分为4组:对照组(等容量生理盐水)、PFOA组(PFOA 10 mg·kg^(-1)·d^(-1))、Mor组(Mor 75mg·kg^(-1)·d^(-1))和PFOA+Mor组(PFOA 10mg·kg^(-1)·d^(-1)+Mor 75mg·kg^(-1)·d^(-1)),每组6只。连续灌胃14d。分别检测血清谷丙转氨酶、谷草转氨酶和碱性磷酸酶的活性,肝组织匀浆中丙二醛、C-反应蛋白、白细胞介素-6的水平,超氧化物歧化酶、过氧化氢酶和环氧化酶-2的活性。结果与对照组比较,PFOA组、PFOA+Mor组小鼠血清谷丙转氨酶、谷草转氨酶、碱性磷酸酶活性及PFOA组小鼠肝组织匀浆中丙二醛、C-反应蛋白、白细胞介素-6水平和环氧化酶-2活性均明显升高,PFOA组、PFOA+Mor组小鼠肝组织匀浆中超氧化物歧化酶、过氧化氢酶活性均明显降低(均P<0.05);与PFOA组比较,Mor组、PFOA+Mor组小鼠血清谷丙转氨酶、谷草转氨酶和碱性磷酸酶活性,肝组织匀浆中丙二醛、C-反应蛋白、白细胞介素-6水平和环氧化酶-2活性均明显降低,肝组织匀浆中超氧化物歧化酶、过氧化氢酶活性均明显升高(均P<0.05)。结论桑色素对PFOA诱导的小鼠肝损伤具有保护作用,其机制可能与抑制氧化应激和炎症反应有关。Objective To investigate the protective effect of morin(Mor) on perfluorooctanoic acid(PFOA)-induced liver injury in mice. Methods Twenty-four male mice were randomly divided into four groups: control group(same volume of normal saline),PFOA group(PFOA 10 mg ?kg'1 ? d_1),M or group(Mor 75 mg ? kg-1 ? d_1) and PFOA + Mor group(PFOA 10 mg ? kg-1 ?d—1+Mor 75 mg ? kg—1 ? d—1 ) , with 6 mice in each group. After treatment for 14 consecutive days, serum activities of alanine transaminase (A L T ), aspartate transaminase (A ST) and alkaline phosphatase( ALP) , hepatic contents of malondialdehyde(MDA) , and hepatic activities of superoxide dismutase (SOD) , catalase (CAT) , C-reactive protein (CRP) , interleukin-6 (IL-6) and cyclooxygenase-2(COX-2) were measured in all groups. Results Compared with control group, liver injury indicators ALT, AST and ALP, inflammatory response markers CRP, IL-6 and COX-2, and lipid peroxidation product MDA increased, but antioxidant enzymes SOD and CAT decreased in PFOA group(P<CO. 05). Compared with PFOA group, simultaneous supplementation of Mor decreased serum activities of ALT, AST and ALP,reduced hepatic contents of MDA,CRP,IL-6 and COX-2, and increased hepatic activities of SOD and CAT(P<C0. 05). Conclusion Mor protects against PFOA-induced liver injury in mice through anti-oxidation and anti-inflammation.

关 键 词：桑色素 全氟辛酸 肝损伤 氧化应激 炎症反应 动物 实验 小鼠 

分 类 号：R33[医药卫生—人体生理学]