氟化物Y500R珊瑚骨羟基磷灰石复合人工骨在兔颅面成骨效应的实验研究  

The osteoplastic effect of Y500R coral hydroxyapatite composite grafts with fluoride on the rabbit skull

作　　者：郭毅[1] 周磊[2] 谢建雅[3] GUO Yi;ZHOU Lei;XIE Jian.ya(Department of Stomatology, Southern Medical University Affiliated Nanhai Hospital,Foshan 528200, China;Guangdong Provincial Stomatological Hospital, Guangzhou 510280, China;Hospital of Traditional Chinese Medicine, FoShan 528000, China)

机构地区：[1]南方医科大学附属南海医院,广东佛山528200 [2]广东省口腔医院.南方医科大学附属口腔医院,广东广州510280 [3]佛山市中医院,广东佛山528000

出　　处：《口腔疾病防治》2016年第12期695-700,共6页Journal of Prevention and Treatment for Stomatological Diseases

基　　金：国家自然科学基金(81170998);广东省医学科研基金(C2012034)

摘　　要：目的探讨氟离子对珊瑚骨羟基磷灰石(coral hydroxyapatite,CHA)在兔颅面成骨效应中的影响,了解结合了氟离子后的珊瑚骨羟基磷灰石成骨效能的变化。方法制备含氟Y500R珊瑚骨羟基磷灰石的人工骨及纯钛金属帽,选用新西兰大白兔24只,分为含氟CHA组和CHA组,每组12只。含氟CHA组兔颅面安放并固定2个装有含氟Y500R珊瑚骨羟基磷灰石人工骨的钛帽,CHA组钛帽装入珊瑚骨羟基磷灰石,注射四环素及钙黄绿素荧光双标记物,分别于2、4、6周时各处死8只实验动物(每组4只),样品处理后作大体标本,观察新骨形成高度和新生骨质荧光带面积,制作硬组织切片行甲苯胺蓝—碱性品红染色,进行组织学观察。结果术后大体标本观察、荧光显微镜观察、硬组织切片染色观察结果显示,术后含氟CHA组新生骨质明显多于CHA组,成骨效应活跃。不同时期新生骨质荧光面积差异具有统计学意义(F=6.36,P=0.019),含氟CHA组明显多于CHA组(P<0.05)。结论低浓度(1.0×10^(-7)mol/L^5.0×10^(-5)mol/L)的氟离子能有效促进骨生成作用。Objective To explore the effect and changes in osteogenesis efficacy of CHA (Coral hydroxyapatite) com?posite grafts with fluoride in guided bone augmentation on rabbit skull. Methods Twenty?four rabbits were studied.Y500R CHA composite grafts contained fluoride and titanium cylinders were prepared. Two titanium cylinders on the testgroup's rabbit skull were filled with composite grafts contain fluoride and non?fluoride grafts were filled in the controlgroup. Cyclomycin and calcein were injected, After 2, 4 and 6 weeks, the animals were sacrificed to obtain ground sec?tions for histology and histomorphometry. The general observation and area of highly fluorescent band of new bone forma?tion was measured after sample treatment. Results Significantly more neogenesis bone was augmented in the test groupsthan the control group. The osteoplastic effect in the test group more athletic. The experimental data had statistical signifi?cance (F = 6.36, P = 0.019 3). Conclusion Low concentration (1.0 × 10?7 mol/L ～ 5.0 × 10?5 mol/L) fluoride can pro?mote bone formation effectively.

关 键 词：氟 珊瑚骨羟基磷灰石 骨代用品 成骨效应 钛帽屏障 

分 类 号：R783.4[医药卫生—口腔医学]