相关炎性因子在脾肾阳虚型溃疡性结肠炎模型大鼠血清和结肠组织的动态表达  

Dynamic Expression of IL-1β,IL-4,IFN-γ and TGFβ1 in Serum and Colon Tissue in Rats with Ulcerative Colitis of Spleen-Kidney Yang Deficiency Type

作　　者：何兰娟[1] 朱向东[1] 王燕[1] 郭婷婷[1] 王迪[1] HE Lan-juan;ZHU Xiang-dong;WANG Yan;GUO Ting-ting;WANG Di(Gansu University of Chinese Medicine, Lanzhou 730000, China)

机构地区：[1]甘肃中医药大学,甘肃兰州730000

出　　处：《中国中医药信息杂志》2017年第1期59-62,共4页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：国家自然科学基金(81360541)

摘　　要：目的观察脾肾阳虚型溃疡性结肠炎模型大鼠血清及结肠组织白细胞介素(IL)-1β、IL-4、干扰素-γ(IFN-γ)及转化生长因子-β1(TGF-β1)表达水平的变化,探讨其在脾肾阳虚型溃疡性结肠炎发生发展过程中的作用。方法采用复合方法建立脾肾阳虚型溃疡性结肠炎大鼠模型。75只Wistar大鼠随机分为空白组、模型3 d组、模型7 d组、模型14 d组和模型21 d组,ELISA检测各组大鼠血清和结肠组织IL-1β、IL-4、IFN-γ及TGF-β1的含量。结果与空白组比较,各模型组大鼠IL-1β、IFN-γ含量明显升高,IL-4、TGF-β1含量明显降低(P<0.05,P<0.01),其中模型7 d组最为明显(P<0.01)。结论促炎因子IL-1β、IFN-γ和抑炎因子IL-4、TGF-β1在脾肾阳虚型溃疡性结肠炎的发病过程中发挥了重要作用。Objective To observe the change rules of expressions of IL-1β, IL-4, IFN-γ and TGF-β1 in serum and colon tissue in rats with ulcerative colitis of spleen-kidney yang deficiency type; To explore the action in the progress of ulcerative colitis of spleen-kidney yang deficiency type. Methods Composite method was used to establish rat models with ulcerative colitis of spleen-kidney yang deficiency type. 75 Wistar rats were randomly divided into blank group, model 3 d group, model 7 d group and model 21 d group. ELISA was used to test the levels of IL-1β, IL-4,IFN-γ and TGF-β1 in serum and colon tissue. Results Compared with the blank group, contents of IL-1β and IFN-γ in model group increased significantly (P<0.05, P<0.01), and the contents of IL-4 and TGF-β1 content decreased (P<0.05, P<0.01),especially in the model 7 d group (P<0.01). Conclusion The pro-inflammatory factor IL-1β, IFN-γ and anti-inflammatory factor IL-4 and TGF-β1 play an important role in rats with ulcerative colitis of spleen-kidney yang deficiency type.

关 键 词：溃疡性结肠炎 脾肾阳虚 白细胞介素-1β 白细胞介素-4 干扰素-γ 转化生长因子-β1 大鼠 

分 类 号：R228[医药卫生—中医基础理论]