东海海域表层沉积物中硫酸盐还原菌分布特征研究  

Distribution of sulfate-reducing bacteria in surface sediments from East China Sea

作　　者：张玉[1,2,3] 贺惠[2,3,4] 米铁柱[1,2,3] 甄毓[1,2,3] 付璐璐[2,3,4] 陈烨[2,3,4] ZHANG Yu;HE Hui;MI Tie-zhu;ZHEN Yu;FU Lu-lu;CHEN Ye(College of Environmental Science and Engineering, Ocean University of China, Qingdao 266100, China;Key Laboratory of Marine Environment and Ecology, Ministry of Education, Qingdao 266100, China;Laboratory for Marine Ecology and Environmental Science, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266071, China;College of Marine Life Science, Ocean University of China, Qingdao 266003, China)

机构地区：[1]中国海洋大学环境科学与工程学院,山东青岛266100 [2]海洋环境与生态教育部重点实验室,山东青岛266100 [3]海洋国家实验室海洋生态与环境科学功能实验室,山东青岛266071 [4]中国海洋大学海洋生命学院,山东青岛266003

出　　处：《中国环境科学》2016年第12期3750-3758,共9页China Environmental Science

基　　金：国家自然科学基金(41521064;41620104001);中国科学院海洋生态与环境科学重点实验室;青岛海洋科学与技术国家实验室海洋生态与环境科学开放基金(KLMEES201601)

摘　　要：利用2011年4、7、8和10月对东海海域4个航次的调查资料,以表层沉积物中硫酸盐还原菌(SRB)为研究对象,针对于SRB所共有的异化型亚硫酸盐还原酶(DSR)中的β亚基基因(dsrB),通过荧光定量PCR技术对SRB丰度的时空分布特征进行了描述.结果表明,SRB丰度变化范围为1.87×105~4.69×108cells/g,平均值为1.15×108cells/g,且4月SRB丰度最低,7月SRB丰度最高;SRB数量在总细菌中的比例介于0.0039%~1.6176%之间,说明SRB在东海表层沉积物的细菌总量中比例很小;SRB丰度的水平分布特征整体表现为南部海域高于北部海域,长江口及浙闽沿岸泥质区高于非泥质区.此外,SRB丰度与环境因子的相关性分析表明,温度和溶解氧是影响SRB丰度的重要因素.Sulfate-reducing bacteria(SRB)are ubiquitous and quantitatively important members in many ecosystems,especially in marine sediments.In this study,the abundance and distribution of SRB was investigated in the surfacesediments from East China Sea in April,July,August and October,2011.A quantitative polymerase chain reaction(qPCR)analysis targeted the dissimilatory sulfite reductaseβsubunit gene(dsrB),which encodes a key enzyme in the sulfatereduction pathway,was performed to assess the abundance of the SRB in the sediments.The abundance of SRB rangedfrom1.87×105to4.69×108cells per gram wet weight sediment,with the lowest and the highest value in April and July,respectively.The ratios of SRB to total bacteria varied from around0.0039%~1.6176%,which implied that SRBconstituted a very small proportion of the total bacteria.The abundance of SRB in the southern areas was higher than thatin the northern areas,and the abundance of SRB in the mud area was much higher than that in the non-mud area.Inaddition,statistical analysis demonstrated that temperature and dissolved oxygen concentration were important factorsaffecting the distribution of SRB abundance.This study presented the temporal and spatial distribution characteristics ofSRB abundance,providing insights into understanding the carbon and sulfur biogeochemical cycles in the surfacesediments from East China Sea.

关 键 词：东海 硫酸盐还原菌 丰度 沉积物 

分 类 号：X172[环境科学与工程—环境科学]