SFE和AMD对光周期诱导菊花成花期芽和叶片蔗糖含量及其相关酶活性的影响  

Effects of SFE and AMD on Sucrose Content and its Relative Enzymes Activities in the Buds and Leaves During Floral Differentiation Under Photoperiodic Induction

作　　者：王文莉[1] 王秀峰[1] 孙宪芝[1] 郑成淑[1] WANG Wen-li;WANG Xiu-feng;SUN Xian-zhi;ZHENG Cheng-shu(Agriculture Ministry Key Laboratory of Horticultural Crop Biolog, State Key Laboratory of Crop Biology, College of Horticulture Science and Engineering/Shandong Agricultural University, Tai’an 271018, China)

机构地区：[1]山东农业大学园艺科学与工程学院,作物生物学国家重点实验室,园艺作物生物学农业部重点开放实验室,山东泰安271018

出　　处：《山东农业大学学报（自然科学版）》2016年第6期807-813,共7页Journal of Shandong Agricultural University：Natural Science Edition

基　　金：教育部留学回国人员科研启动基金(33206)

摘　　要：以切花菊品种‘神马’为试材,研究了蔗糖合成酶促进剂SFE和抑制剂AMD对光周期诱导菊花成花过程芽和叶片中蔗糖含量及蔗糖代谢关键酶——蔗糖合成酶(SS)和蔗糖磷酸合成酶(SPS)活性的的影响。结果表明,SFE和AMD处理及对照叶片和芽中蔗糖含量在花芽分化启动期(II)均显著升高,分化启动后降低,其中AMD处理的上升幅度明显小于其它二者;SFE处理的叶片蔗糖含量在花瓣分化之前高于对照,AMD处理的则比对照及SFE处理的减少;芽中蔗糖含量始终低于叶片,但整个分化过程特别是中后期对照和SFE处理的芽中增幅高于叶中。SFE处理与对照叶片和芽中SPS和SS活性均随花芽分化启动有所增强,以叶片中SPS活性增幅最大,高达80.6%,比同期对照高26.8%;叶中SPS和SS活性高于芽中;分化启动后逐渐降低,但整个分化过程始终高于处理前水平;不同分化阶段SPS和SS活性变化与对照不尽相同,SS活性从处理至总苞鳞片分化期(III)持续升高达到峰值,而后下降在小花原基分化后期(V)与对照接近。AMD处理植株叶片和芽中SPS和SS活性自处理开始均有所下降,在花芽分化启动期(II)叶片SPS活性下降最多,达29.6%,芽中SS活性在III期降至最低,为分化前的33.7%,之后缓慢回升,至花瓣分化后期(VII)与其它二者接近。SFE和AMD处理对菊花花芽分化进程有影响,SFE处理的植株花芽分化启动和结束时间分别比对照提前1 d和2 d,AMD处理的则分别比对照推迟3 d和6 d。分析表明,AMD和SFE通过调节SPS和SS活性影响蔗糖合成进而影响菊花花芽分化进行.Effects of SFE and AMD on the sucrose content and the activities of sucrose metabolizing enzymes --- sucrose phosphate synthase (SPS) and sucrose synthase (SS) in the buds and leaves of cut flower chrysanthemum (Dendranthema grandiflorium ‘Shenma’) were studied during floral differentiation under photoperiodic inducement. The results showed that the contents of sucrose in the buds and leaves of SFE and AMD treated plants together with control plants were increased significantly at the stage of initial differentiation (II), while the increase of AMD treated plants was lower than the other two.The sucrose contents decreased after differentiation, but which were always higher than that of before treatment. The sucrose content in leaves of SFE treated plants was higher than that of the control before the stage of petal differentiation, while it decreased in AMD treated plants compare with the control and SFE treated plants. Sucrose content in buds was always lower than that in leaves, but the increase in buds is bigger than that in leaves through the whole differentiation process especially the midanaphase in the control and SFE treated plants. The activities of sucrose phosphate synthase (SPS) and sucrosesynthase (SS) in leaves and buds of the control and SFE treated plants were increased slightly after initial differentiation. Thelargest increase in leaves is up to 80.6% and it was 26.8% higher than the control at same stage. The activities of SPS and SSin leaves is higher than that in bud, then they decreased gradually after differentiation, but which was always higher than thatof before treatment during the whole floral differentiation stage. The activities of SPS and SS are not the same as the control in different stages of differentiation. The activity of SS increased constantly from the beginning to the stage of involucre primordial differentiation (III) and reached a peak, then decreased and approached to the control at the later stage of floret primordium differentiation (V). The activities of SPS and SS

关 键 词：菊花 花芽分化 SFE和AMD 蔗糖 蔗糖酶 

分 类 号：S682.11[农业科学—观赏园艺]