胆囊癌组织中TROP2、PTEN和p-AKT的表达及其临床意义  

Clinical value of TRo P2, PTEN and p-AKT expression in gallbladder carcinoma

作　　者：滕世峰 李新星[1] 张言言 张卫刚[1] 张宪文[1] 姚骏[1] 姚厚山[1] 黄歆[1] 胡志前[1] TENG Shifeng;LI Xin-xing;ZHANG Yan-yan;ZHANG Wei-gang;ZHANG Xian-wen;YAO Jun;YAO Hou-shan;HUANG Xin;HU Zhi-qian(Department of General Surgery, Changzheng Hospital, the Second Military Medical University Shanghai 200003, China)

机构地区：[1]第二军医大学附属长征医院普外一科,上海200030

出　　处：《肝胆胰外科杂志》2017年第1期46-50,共5页Journal of Hepatopancreatobiliary Surgery

基　　金：国家青年科学基金项目(8140101730)

摘　　要：目的探讨人滋养层细胞表面抗原-2(human trophoblast cell-surface antigen 2,TROP2)、磷酸酶与张力蛋白同源物(phosphatase and tensin homolog,PTEN)、磷酸化蛋白激酶B(phosphorylated protein kinase B,p-AKT)在胆囊癌组织中的表达情况、相互关系及临床意义。方法收集我院2003年1月至2009年12月期间确诊并手术的胆囊癌患者71例,获得胆囊癌组织标本71例及癌旁组织15例。采用免疫组织化学法检测TROP2、PTEN和p-AKT的表达,χ~2检验分析TROP2、PTEN和p-AKT蛋白表达水平与临床病理参数之间的关系,Spearman检验法分析TROP2、PTEN和p-AKT间两两相关性。结果 (1)与癌旁组织比,TROP2和p-AKT蛋白在胆囊癌组织中的阳性表达率明显升高(P<0.05),而PTEN蛋白在胆囊癌组织中的阳性表达率明显降低(P<0.05);(2)TROP2、PTEN和p-AKT三种蛋白的表达与胆囊癌患者的性别、年龄均无关(P>0.05),但与胆囊结石、分化程度、其他脏器侵犯、浸润深度及TNM分期相关(P<0.05);(3)TROP2与p-AKT呈正相关(P<0.05),但两者与PTEN呈负相关(P<0.05)。结论 TROP2/PTEN/p-AKT信号通路异常激活可能是胆囊癌恶性进展的重要原因,其机制可能涉及TROP2对PTEN及p-AKT的调控作用,这为临床上靶向干预胆囊癌研究提供理论基础。Objective To explore the relationship and clinical significance of human trophoblast cell-surface antigen 2 (TROP2), phosphatase and tensin homolog (PTEN), phosphorylated protein kinase B (p-AKT) in gallbladder carcinoma. Methods A tatal of 71 patients with gallbladder carcinoma in our hospital were selected between Jan. 2003 and Dec. 2009. Immunohistochemical staining method was used to detected the protein expression of TROP2, PTEN and p-AKT in 71 cases of gallbladder carcinoma tissues and 15 cases of tumor adjacent tissues, Pearson chi-square test was used to analyze the relationship between the protein expression of TROP2,PTEN, p-AKT and the clinical pathological parameters, Spearman test was used to detect the relationship among TROP2, PTEN and p-AKT. Results (1)Compared with the tumor adjacent tissues, the protein expression of TROP2 and p-AKT in gallbladder carcinoma tissues were significantly increased (P<0.05), while the protein expression of PTEN in gallbladder carcinoma tissues was significantly decreased (P<0.05); (2)The proteins expression of TROP2, PTEN and p-AKT were independent with the gender and age (P>0.05), but which was correlated with gallstones, tumor differentiation, organ invasion, depth of invasion and TNM stage (P<0.05); (3)The protein expression of TROP2 was positively correlated with p-AKT (P<0.05), but which was negatively corelated with PTEN (P<0.05). Conclusion Abnormal activation of TROP2/PTEN/p-AKT signal pathway may play an important role in malignant progression of gallbladder carcinoma, the mechanism may be related to TROP2 negative regulation of PTEN expression, inhibiting its dephosphorylation, resulting in increased expression of p-AKT, and promoting the proliferation and metastasis of gallbladder cancer cells. It is an important cause for malignant progression of gallbladder carcinoma, which provides a theoretical basis for clinical targeted intervention studies.

关 键 词：胆囊癌 人滋养层细胞表面抗原-2 磷酸酶与张力蛋白同源物 磷酸化蛋白激酶B 免疫组化分析 预后 

分 类 号：R735.8[医药卫生—肿瘤]