源自黄斑篮子鱼肠道菌群的B30菌株降解浒苔多糖的活性研究  

A Study of Enteromorpha Polysaccharides-Degraded Ability of Strain B30 from Siganus Oramin Intestina

作　　者：谢罗瀚 韩学凤[2] 张志标[2] 李进[2] 张青岩 钟名其[2] 胡忠[2] XIE Luo-Han;HAN Xue-Feng;ZHANG Zhi-Biao;LI Jin;ZHANG Qing-Yan;ZHONG Ming-Qi;HU Zhong(Jinshan Middle School of Shantou, Shantou, Guangdong, 515073;Biology Department of Shantou University, Shantou, Guangdong, 515063)

机构地区：[1]汕头市金山中学,广东汕头515073 [2]汕头大学生物系,广东汕头515063

出　　处：《韩山师范学院学报》2016年第6期49-55,共7页Journal of Hanshan Normal University

基　　金：广东省自然科学基金项目(项目编号:S2011030005257)

摘　　要：以浒苔多糖作为唯一碳源,从黄斑篮子鱼肠道菌群筛选得到一株能够降解浒苔多糖的菌株,经16S rDNA鉴定为Vibrio sp.B30;其产浒苔多糖降解酶的最适条件为培养时间24 h、温度30℃、起始p H值为7、最适氮源为硫酸铵.该酶的最适反应催化温度为50℃、pH值为7,并且10 mmol/L的钙离子或钠离子能够较明显提高该酶酶活力.0.5 g/L和1 g/L的NP40、聚氯乙烯月桂醚、Tween80、Tween20、Trition X-100表面活性剂对浒苔多糖降解酶活力具有一定的提高作用.An Enteromorpha polysaccharides-degrading strain Vibrio sp. B30 is isolated from Siganus oramin intestinal using E. polysaccharides as the sole carbon source. The optimum fermentation conditions for producing E. polysaccharides lyase are cultivated 24h at 30℃, the initial pH 7, and the most suitable nitrogen source is ammonium sulfate. The E. polysaccharides lyase can be enhanced by Ca2 + , Na+ and surfactant NP40,Tween80, Tween20, TritionX-100. Vibrio sp. B30 from S. oramin intestinal can use E. polysaccharides as the sole carbon source.

关 键 词：浒苔多糖 降解 Vibriosp.B30 黄斑篮子鱼肠道菌群 

分 类 号：X172[环境科学与工程—环境科学]