南极德雷克海峡表层海水酵母菌多样性研究  

Phylogenetic Diversity of Culturable Yeast from Surface Seawater of the Drake Passage in Antarctic

作　　者：李昭[1] 王伟[1] 郝建华[1] 孙谧[1] LI Zhao;WANG Wei;HAO Jian-hua;SUN Mi(Key Laboratory of Sustainable Development of Marine Fisheries, Yellow Sea Fisheries Research Institute Chinese Academy of Fishery Sciences,Qingdao 266000, PRC)

机构地区：[1]中国水产科学研究院黄海水产研究所,农业部海洋渔业可持续发展重点实验室,山东青岛266000

出　　处：《湖南农业科学》2017年第1期68-72,共5页Hunan Agricultural Sciences

基　　金：中国水产科学研究院基本科研业务费(2016HY-ZD0902-3);国家实验室--鳌山科技计划(2015ASKJ02-06);青岛市博士后研究人员应用研究项目资助(NO.Q51201407)

摘　　要：为了研究德雷克海峡的微生物多样性,采集南极德雷克海峡表层海水,通过传统分离培养法获得可培养的酵母菌株,对其18S r DNA保守序列进行测序及系统发生分析。结果表明:共分离出45株酵母菌,属于9个属,12个种;其中,优势属为Meyerozyma属,优势种为Meyerozyma guilliermondii。对所有菌株的过氧化氢酶活性进行检测,发现23株酵母菌具有强烈的过氧化氢酶活性,13株具有弱过氧化氢酶活性,即可分解过氧化氢的菌株占总菌株数的80%。In order to study microbial diversity in the surface seawater of the Drake Passage in Antarctic, 7 surface seawater samples were collected to isolate the strains of culturable yeast by the traditional method, and 18S rDNA sequencing and phylogenetic analyses were carried out. The results showed that 45 yeast strains were isolated and there were 9 genera and 12 species. Meyerozyma sp. and Meyerozyma guilliermondii was the dominant genera and species. The results of the catalase activities showed that 23 strains were strong catalase positive, 13 strains were weak, and 80% strains were catalase positive.

关 键 词：可培养酵母菌 18SrDNA 过氧化氢酶 

分 类 号：Q938.8[生物学—微生物学]