松乳菇多糖刺激免疫细胞增殖及诱导肿瘤细胞凋亡的研究  

Polysaccharide from Lactarius deliciosus Gray Stimulates Immunocyte Proliferation and Induces Tumor Cells Apoptosis

作　　者：钱叶[1] 丁祥[1] 曾益春[2] 伏雷 朱洪庆[1] 张楠[1] 侯万儒[1] 侯怡铃[1] QIAN Ye;DING Xiang;ZENG Yichun;FU Lei;ZHU Hongqing;ZHANG Nan;HOU Wanru;HOU Yiling(Key Laboratory of Southwest China Wildlife Resources Conservation, Ministry of Education,College of Life Science, China West Normal University, Nanchong 637009, China;Sericultural Research Institute, Sichuan Academy of Agricultural Sciences, Nanchong 637000, China)

机构地区：[1]西华师范大学生命科学学院,西南野生动植物资源保护省部共建教育部重点实验室,四川南充637009 [2]四川省农业科学院蚕业研究所,四川南充637000

出　　处：《食品科学》2017年第5期220-226,共7页Food Science

基　　金：国家自然科学基金青年科学基金项目(31400016)

摘　　要：为研究松乳菇多糖(Lactarius deliciosus Gray polysaccharide,LDG-A)对特异性免疫细胞的增殖效应活性及对肿瘤细胞凋亡的影响,在LDG-A作用下检测T淋巴细胞、B淋巴细胞的增殖效应,并观察T淋巴细胞、B淋巴细胞的细胞形态,检测T淋巴细胞、B淋巴细胞在LDG-A的作用下处于细胞周期各个时期的细胞数量及B淋巴细胞分泌抗体的情况,观察LDG-A作用于肺癌细胞A549的凋亡和细胞骨架情况。结果表明:随着LDG-A质量浓度的增加,能够促进T淋巴细胞、B淋巴细胞的增殖,并呈现出一定的剂量依赖关系;同时LDG-A能够减少T淋巴细胞、B淋巴细胞在G0/G^1期的细胞数量百分比,促进细胞进入G^2/M期,具有促进B淋巴细胞分泌抗体免疫球蛋白M、免疫球蛋白D和免疫球蛋白E的作用。在12.5~50.0μg/m L范围内,LDG-A能够诱导肺癌细胞A549凋亡,并呈现出一定的剂量依赖关系;在细胞凋亡时F-肌动蛋白丝断裂,蛋白纤维网络结构被破坏。综上所述,在体外实验中,LDG-A对特异性免疫细胞具有促增殖效应活性,能够促进肿瘤细胞的凋亡。To understand the effect of Lactarius deliciosus Gray polysaccharide(LDG-A)on the proliferation of specific immunocytes and tumor cell apoptosis,we tested and observed the proliferation and morphological changes of Tlymphocytes and B lymphocytes in the presence of LDG-A,determined the number of T lymphocytes and B lymphocytes in each stage of the cell cycle,and also evaluated antibody secretion by B lymphocytes under the stimulation of LDG-A.In addition,the apoptosis and cytoskeleton of lung cancer cell A549were evaluated.The results showed that the proliferation of B lymphocytes and T lymphocytes’proliferation was promoted LDG-A in a dose-response manner.LDG-A could also reduce the number of B lymphocytes at the G0/G1phase and promote them to enter the G2/M phase.Meanwhile,LDG-A also promoted the secretion of IgM,IgD and IgE by B lymphocytes.In addition,LDG-A in the range of12.5–50.0μg/mL could induce the apoptosis of lung cancer cell A549in a dose-dependent manner.Moreover,we observed fractured F-actin filaments in the cells as well as damaged protein fiber networks during apoptosis.In conclusion,LDG-A has a promoting effect on the proliferation of specific immunocytes,and accelerates the apoptosis of tumor cells in vitro.

关 键 词：松乳菇多糖 免疫细胞 增殖效应 肿瘤细胞 凋亡 

分 类 号：Q939.91[生物学—微生物学]