毛细管电泳-电化学发光法测定荷叶中荷叶碱与莲子心中莲心碱含量  

Determination of Nuciferine in Nelumbinis Folium and Liensinine in Nelumbinis Plumula Using Capillary Electrophoresis Coupled with Electrochemilumolinescence Method

作　　者：罗珍连 邓光辉[1] LUO Zhenlian;DENG Guanghui(Guangxi Key Laboratory of Chemistry and Engineering of Forest Products, Key Laboratory of Chemical and Biological Transformation Process of Guangxi Higher Education Institutes, Guangxi Key Laboratory Cultivation Base for Polysaccharide Materials and their Modification, School of Chemistry and Che)

机构地区：[1]广西民族大学化学化工学院,广西林产化学与工程重点实验室,广西高校化学与生物转化过程新技术重点实验室,广西多糖材料与改性重点实验室培育基地,广西南宁530006

出　　处：《食品科学》2017年第6期197-201,共5页Food Science

基　　金：广西壮族自治区自然科学基金项目(0640038);国家自然科学基金地区科学基金项目(21365004;21065001);广西高校科学技术研究重点项目(2D2014041);广西高校人才小高地建设创新团队项目(桂教[2011]47)

摘　　要：建立毛细管电泳-电化学发光法同时测定荷叶中荷叶碱与莲子心中莲心碱含量的方法。对三联吡啶钉(Ru(bpy)_3^(2+))溶液浓度、检测电位、磷酸盐缓冲液浓度和pH值、进样时间和分离电压等实验条件进行考察和优化。结果表明,在检测电位为1.20 V,运行缓冲液为10 mmol/L磷酸盐缓冲液(pH 5.74),5 mmol/L Ru(bpy)_3^(2+)溶液,检测池内磷酸盐缓冲液浓度为60 mmol/L(pH 8.30),进样电压为8 kV,进样时间为10 s,分离电压为13 kV,荷叶碱检出限为7.7×10^(-7)mol/L(R_(SN)-3),莲心碱检出限为7.8×10^(-7)mol/L(R_(SN)-3)。对浓度为6.8×10^(-5)mol/L荷叶碱和3.1×10^(-5)mol/L莲心碱的标准品溶液进行5次平行测定表明:荷叶碱峰面积的相对标准偏差(relative standard deviation,RSD)为3.76%,迁移时间RSD为0.83%;莲心碱峰面积RSD为4.28%,迁移时间RSD为1.37%。该方法可用于测定荷叶中的荷叶碱与莲子心中莲心碱含量。A new method was developed for the determination of nuciferine in Nelumbinis Folium and liensinine inNelumbinis Plumula by capillary electrophoresis coupled with electrochemilumolinescence(ECL)detection.The optimizedexperimental conditions were determined as follows:Ru(bpy)32+concentration,5mmol/L;detection potential,1.20V;10mmol/L phosphate buffer(pH5.74)as running buffer,60mmol/L(pH8.30)phosphate buffer contained in the detectionreservoir;injection time,10s;separation voltage,13kV.Detection limits(LOD)of7.7×10-7mol/L(RSN=3)for nuciferineand7.8×10-7mol/L(RSN=3)for liensinine were obtained.Relative standard derivations of electrophoretic peak area andmigration time were3.76%and0.83%for6.8×10-5mol/L nuciferine and4.28%and1.37%for3.1×10-5mol/L liensininefrom five replicate determinations,respectively.The method can be applied to detect nuciferine in lotus leaves and liensininein Nelumbinis Plumula with satisfactory results.

关 键 词：毛细管电泳 电化学发光 荷叶碱 莲心碱 

分 类 号：O657.8[理学—分析化学]