大肠杆菌对钯(Ⅱ)的吸附机理研究  

Study on the Adsorption Mechanism of Pd(Ⅱ) on Escherichia coli Biomass

作　　者：刘新星[1] 何佳[1] 董海刚[2] 谭玲[1] 何敏[1] 郭子文[3] 谢建平[1] LIU Xinxing;HE Jia;DONG Haigang;TAN Ling;HE Min;GUO Ziwen;XIE Jianping(Key Laboratory of Biohydrometallurgy, Ministry of Education, School of Mineral Processing and Bioengineering, Central South University, Changsha 410083, China;State Key Laboratory of Advanced Technologies for Comprehensive Utilization of Platinum Metals, Kunming Institute of Precious Metals, Kunming 650106, China;College of Life Sciences, Inner Mongolia Agricultural University, Hohhot 010010, China)

机构地区：[1]中南大学资源加工与生物工程学院,教育部生物冶金重点实验室,长沙410083 [2]昆明贵金属研究所稀贵金属综合利用新技术国家重点实验室,昆明650106 [3]内蒙古农业大学生命科学学院,呼和浩特010010

出　　处：《贵金属》2017年第1期75-82,共8页Precious Metals

基　　金：国家自然科学基金(51104189);中国博士后科学基金特别资助(2015T80880)、中国博士后科学基金(2013M531814);中南大学博士后基金;稀贵金属综合利用新技术国家重点实验室开放课题(SKL-SPM-201508);粉末冶金国家重点实验室开放课题

摘　　要：用FTIR、XPS、TEM等表征方法对溶液中大肠杆菌吸附钯(Ⅱ)的机理行了研究。结果表明,该吸附过程与大肠杆菌和钯(Ⅱ)间静电作用有关,在pH为2.0时吸附量最大,可达120.08 mg/g;对大肠杆菌进行化学修饰,FTIR结果表明细胞表面的氨基、羧基可能为吸附钯(Ⅱ)的主要基团,涉及到表面络合机制;TEM及XPS结果显示吸附6 h后菌体内有尺寸为5~15 nm的钯纳米颗粒生成,说明该吸附过程还存在还原反应。大肠杆菌对钯(Ⅱ)的吸附过程是静电作用、表面络合、氧化还原等机制共同作用的结果。The adsorption mechanism of Pd(II)on Escherichia coli was investigated by using FTIR,XPS,TEM and other characterization methods.The results revealed that the adsorption process was related tothe electrostatic interaction between E.coli and Pd(II)with a maximum adsorption capacity of120.08mg/g at pH2.0.After the chemical modification of E.coli,FTIR results showed that the amino andcarboxyl groups on the cell surface may be the major groups for binding Pd(II),suggesting a surfacecomplexation mechanism involved.TEM and XPS results showed that palladium nanoparticles with a sizeof5~15nm were deposited on E.coli,indicating the existence of reduction process.Therefore,it can beconcluded that several mechanisms such as electrostatic adsorption,surface complexation andbioreduction,are involved in the biosorption process of Pd(II)on E.coli.However,the confirmation of theelectron donor and the site of the biological reduction needs further research.

关 键 词：微生物冶金 大肠杆菌 钯(II) 吸附机理 

分 类 号：TF836[冶金工程—有色金属冶金] X172[环境科学与工程—环境科学]