Rab11-FIP4对结肠癌细胞生物学特性的影响  

The influence of Rab11-FIP4 in cytological characteristics of colorectal cancer

作　　者：杨守醒 俞耀军[2] 王建嶂[1] 郑波[1] 薛战雄[1] 徐昌隆[1] YANG Shouxing;YU Yaojun;WANG Jianzhang;ZHENG Bo;XUE Zhanxiong;XU Changlong(Department of Gastroenterology the Second Affiliated Hospital & Yuying Children’s Hospital of Wenzhou Medical University, Wenzhou, 325027;Department of Gastrointestinal Surgery, the Second Affiliated Hospital & Yuying Children’s Hospital of Wenzhou Medical University, Wenzhou, 325027)

机构地区：[1]温州医科大学附属第二医院育英儿童医院消化内科,浙江温州325027 [2]温州医科大学附属第二医院育英儿童医院胃肠外科,浙江温州325027

出　　处：《温州医科大学学报》2017年第3期174-177,共4页Journal of Wenzhou Medical University

基　　金：浙江省自然科学基金资助项目(LY16H160055);温州市科技计划项目(Y20150156)

摘　　要：目的:探讨Rab11-FIP4的表达变化对结肠癌细胞生物学特性的影响。方法:采用结肠癌细胞株HCT116转染Rab11-FIP4特异性siR NA干扰Rab11-FIP4表达。用Western blot实验检测干扰效率。运用CCK-8实验和Transwell实验分别检测干扰Rab11-FIP4对结肠癌细胞株HCT116增殖和运动能力的影响。结果:siR NA转染使Rab11-FIP4表达量从100.2±21.5降低至23.5±5.8,差异有统计学意义(P<0.01)。干扰Rab11-FIP4后结肠癌细胞株HCT116的体外增殖明显下降,第2天的抑制率为29.40%,差异无统计学意义(P<0.05),第3天的抑制率为37.65%,差异有统计学意义(P<0.05),结肠癌细胞的迁移数从193.4±22.1降至113.9±12.7,差异有统计学意义(P<0.05),细胞侵袭数从113.3±16.7降至66.5±6.7,差异有统计学意义(P<0.05)。结论:干扰Rab11-FIP4的表达能抑制结肠癌细胞的体外增殖、迁移和侵袭能力,Rab11-FIP4可能在结肠癌的进展中发挥重要作用。Objective: To investigate the effects of Rab11-FIP4 on cytological characteristics of colorectal cancer (CRC). Methods: Western blot was used to detect the expression of Rab11-FIP4 in CRC cell line HCT116,which was transfected with siRNAs that specifically targeting Rab11-FIP4. Proliferation and migration of HCT116 cells were analyzed by CCK-8 assay and Transwell assay. Results: Rab11-FIP4 by siRNA transfection expressing in 100.2±21.5 reduced to 23.5±5.8 (P<0.01). Interference Rab11-FIP4, CRC cell line HCT116 in vitro,proliferation decreased obviously, the second day of the inhibition rate was 29.40%, the inhibition rate of 37.65% on the third day (P<0.05), number of cells migrating from 193.4±22.1 to 113.9±12.7 (P<0.05) and cell invasion number from 113.3±16.7 to 66.5±6.7 (P<0.05). Conclusion: Knockdown of Rab11-FIP4 significantly inhibits proliferation, migration and invasion of CRC cells in vitro, indicating its important role in CRC progression.

关 键 词：Rab11-FIP4 结肠肿瘤 RNA 小分子干扰 细胞增殖 细胞运动 肿瘤侵润 

分 类 号：R735.3[医药卫生—肿瘤]