CD117和Sox10在恶性黑色素瘤中的表达及意义  

Expression and significance of CD117 and Sox10 in malignant melanoma

作　　者：温莉虹 林瑞泼[2] 陈阿德[3] 黄兆浪 郑向阳[1] 李秧秧[4] 黄卡特[4] 陈国荣[4] WEN Lihong;LIN Ruipo;CHEN Ade;HUANG Zhaolang;ZHENG Xiangyang;LI Yangyang;HUANG Kate;CHEN Guorong(Department of Pathology, the Affiliated Cangnan Hospital of Wenzhou Medical University, Wenzhou, 325800;Department of Science and Education, the Affiliated Cangnan Hospital of Wenzhou Medical University, Wenzhou, 325800;Department of Clinical Laboratory, the Affiliated Cangnan Hospital of Wenzhou Medical University, Wenzhou,325800;Department of Pathology, the First Affiliated Hospital of Wenzhou Medical University, Wenzhou,325015)

机构地区：[1]温州医科大学附属苍南医院病理科,浙江温州325800 [2]温州医科大学附属苍南医院科教科,浙江温州325800 [3]温州医科大学附属苍南医院检验科,浙江温州325800 [4]温州医科大学附属第一医院病理科,浙江温州325015

出　　处：《温州医科大学学报》2017年第3期197-200,共4页Journal of Wenzhou Medical University

基　　金：苍南县科技计划项目(2015S05);温州市科技局科研基金资助项目(Y20130001);浙江省科技厅国际合作项目(2013C24031)

摘　　要：目的:分析黏膜、肢端和非肢端皮肤恶性黑色素瘤(MM)中CD117和Sox10表达以及c-kit基因扩增情况,探讨其与临床病理的关系。方法:PCR法检测黏膜、肢端和非肢端MM中c-kit基因的扩增量,并以色素痣作为对照。免疫组织化学法检测黏膜、肢端和非肢端MM中CD117的表达量,并与色素痣进行对照。免疫组织化学法检测黏膜、肢端和非肢端MM中Sox10、S-100和HMB45的表达,比较分析各组阳性细胞数及总的阳性率差异。结果:黏膜、肢端和非肢端MM中c-kit基因扩增量显著大于色素痣,差异有统计学意义(P<0.05)。黏膜、肢端、非肢端MM和色素痣中CD117阳性率分别为78.5%、73.6%、73.3%和25.0%,3组MM阳性率均高于色素痣(P<0.05)。3组MM中Sox10的阳性细胞数均高于S-100和HMB45,差异有统计学意义(P<0.05);Sox10、S-100和HMB45在MM中的阳性率分别为100.0%、87.5%和70.8%,差异有统计学意义(P<0.05)。结论:c-kit基因扩增及CD117蛋白表达水平增高不仅可作为MM与色素痣的鉴别诊断标志,同时也为MM的靶向治疗提供靶点;Sox10阳性率可作为黑色素细胞起源的肿瘤诊断标志。Objective: To analyze the value of CD117, Sox10 expression and c-kit gene amplification in mucous, acral and non-acral melanoma and explore the relationship between it and clinical pathology. Methods:The gene levels of c-kit in mucosal, acral and non acral melanoma were detected by PCR, with pigmented nevus as control. The protein expressions of CD117, sox10, 1-100 and HMB45 were detected by immunohistochemistry.Results: The expression of c-kit gene from the three MM groups was higher than that of pigmented nevus (P<0.05). The immunohistochemical results demonstrated that the number of positive cells of CD117 from the three MM groups was marginally higher than that of pigmented nevus (P<0.05). The number of positive cells of Sox10 in the three MM groups was higher than that of S-100 and HMB45 (P<0.05). The positive rate of CD117 in mucosal, acral and non acral MM was 78.5%, 73.6% and 73.3% respectively, while 25% in pigmented nevus control (P<0.05). The positive rate of Sox10, S-100 and HMB45 in MM was 100.0%, 87.5% and 70.8% respectively (P<0.05). Conclusion: C-kit gene amplification and its downstream transcription protein CD117 enhanced positive expression can be applied as clinical potential therapy target. The positive rate of Sox10 is higher than S-100 and HMB45, and may be applied as tumor differentiation marker for origins of melanocytes.

关 键 词：黑色素瘤 CD117 C-KIT Sox10 免疫组织化学 

分 类 号：R365[医药卫生—病理学]