空间位阻对溶解态甲基菲生物降解的影响  

Effect of steric hindrance on the biodegradation of dissolved methyl-phenanthrene

作　　者：杜兰[1] 张振轩[1] 朱亚先[2] 张勇 DU Lan;ZHANG Zhen-xuan;ZHU Ya-xian;ZHANG Yong(State Key Laboratory of Marine Environmental Science of China, College of Environment and Ecology, Xiamen University, Xiamen 361102, China;Department of Chemistry, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China)

机构地区：[1]厦门大学环境与生态学院,近海海洋环境科学国家重点实验室,福建厦门361102 [2]厦门大学化学化工学院化学系,福建厦门361005

出　　处：《中国环境科学》2017年第4期1375-1379,共5页China Environmental Science

基　　金：国家自然科学基金资助项目(21177102,21577110);国家海洋局海洋溢油鉴别与损害评估技术重点实验室开放基金资助项目(201405);高等学校博士学科点专项科研基金资助项目(20130121130005)

摘　　要：以菲(Phe)和甲基菲(MP:1-MP、3-MP、4-MP、9-MP)为研究对象,运用同步焚光法研究微生物Novosphingobium pentaromativorans US6-1对5种溶解态多环芳烃(PAHs)的降解特性.结果显示:MP在8×10^(-7),1.2×10^(-6)mol/L2种浓度下,其生物降解过程符合零级动力学,降解速率均为:4-MP<9-MP<1-MP<3-MP;5种PAHs的最大生物降解速率同理论最大跨膜通量存在良好的线性关系;NaN_3对Phe降解速率影响较小,但抑制MP降解且抑制程度与取代基位置有关.The biodegradation of dissolved phenanthrene(Phe)and methyl-phenanthrene(MP),including1-MP,3-MP,4-MP and9-MP by the bacterium Novosphingobium pentaromativorans US6-1was individually studied using synchronous fluorescence spectrometry.The biodegradation of MP was adequately described by zero-order kinetics at two nconcentrations,i.e.,8×10-7mol/L and1.2×10-6mol/L.The rates of biodegradation were generally in the following order:4-MP<9-MP<1-MP<3-MP.There was a good linear relationship between the measured maximum biodegradation rates and the theoretical maximum transmembrane fluxes.The addition of NaN3exerted little effects on the biodegradation of Phe but inhibited that of MP,with the degree of inhibition dependent on the position of the methyl substituent.

关 键 词：甲基菲 同系物 生物降解 跨膜过程 

分 类 号：X127[环境科学与工程—环境科学]