单纯疱疹病毒1型感染神经胶质细胞的代谢组学研究  

Study of the metabolomics of glia cells infected by herpes simplex virus type 1

作　　者：马丽华[1] 杨宏静[1] 徐晓艳[1,2] 石中全[1] 邓榕[3] Ma Lihua;Yang Hongjing;Xu Xiaoyan;Shi Zhongquan;Deng Rong(Department of Basic Medicine, Chongqing Three Gorges Medical College, Chongqing 404120, China;Department of Neurology Chongqing Three Gorges Central Hospital, Chongqing 404120, China;Institute of Neuroscience, Chongqing Medical University Chongqing 400016, China)

机构地区：[1]重庆三峡医药高等专科学校基础医学部,重庆404120 [2]重庆医科大学神经科学研究中心,重庆400016 [3]重庆三峡中心医院神经内科,重庆404120

出　　处：《中国组织化学与细胞化学杂志》2017年第2期134-141,共8页Chinese Journal of Histochemistry and Cytochemistry

基　　金：重庆市教委科学技术研究项目资助(KJ1402507)

摘　　要：目的研究单纯疱疹病毒1型(HSV-1)对人类少突胶质细胞(OL细胞)代谢的影响,从而探讨HSV-1感染的分子机制。方法将OL细胞随机分为两组,一组为感染组,感染HSV-1,一组为正常对照组,不感染HSV-1,运用核磁共振氢谱(~1H NMR)检测二组细胞培养液水相代谢产物,最后由偏最小二乘法-判别分析(PLS-DA)对数据进行分析。结果感染组与正常对照组细胞培养液水相代谢物的组成有显著差异:被HSV-1感染的OL细胞的培养液中,亮氨酸、谷氨酸、谷氨酰胺增多,丙氨酸、甘氨酸、组氨酸、异亮氨酸、乳酸减少。结论 HSV-1感染改变OL细胞的氨基酸代谢,HSV-1可能通过扰乱宿主细胞的代谢路径,促进自己的繁殖,并影响神经系统的功能活动。Objective To investigate the effect of herpes simplex virus type1(HSV-1)infection on the metabolism of human oligodendroglia cells(OL cells)and explore the molecular mechanism of HSV-1infection.Methods OL cells were randomly divided into two groups,the HSV-1infection group and the normal control group.The metabolites in the aqueous phase of the culture media were extracted and detected by proton nuclear magnetic resonance spectroscopy(1H NMR).The data were analyzed by partial least square discriminant analysis(PLS-DA).Results The metabolic profiles of the infection and normal control groups varied significantly.In the culture medium of the infection group,the contents of leucine,glutamate and glutamine increased,while those of alanine,glycine,histidine,isoleucine and lactate decreased.Conclusion HSV-1infection interferes with the amino acid metabolism of OL cells.HSV-1may impact the metabolic pathway of its host cells to facilitate its own proliferation.It also affects the normal function of the nervous system.

关 键 词：单纯疱疹病毒1型 代谢组学 核磁共振氢谱 神经胶质细胞 

分 类 号：R3[医药卫生—基础医学]