C8-oxo-HSl对P.aeruginosa在污水处理中生长特性影响  

Effect of C_8-oxo-HSL on the growth of P. aeruginosa in wastewater treatment

作　　者：周礼杰[1,2,3] 王鑫[3] 韩倩 朱婷婷 成功 夏四清[3] ZHOU Li-jie;WANG Xin;HAN Qian;ZHU Ting-ting;CHENG Gong;XIA Si-qing(State Environmental Protection Key Laboratory of Drinking Water Source Management and Technology, Shenzhen Key Laboratory of Drinking Water Source Safety Control, Shenzhen Key Laboratory of Emerging Contaminants Detection & Control in Water Environment, Shenzhen Academy of Environmental Sciences,;College of Chemistry and Emvironmental Engineering, Shenzhen University, Shonzhen 518060, China;State Key Laboratory of Pollution Control and Resource Reuse, College of Environmental Science and Engineering, Tongji University, Shanghai 200092, China)

机构地区：[1]深圳市环境科学研究院,国家环境保护饮用水水源地管理技术重点实验室,深圳市饮用水水源地安全保障重点实验室,深圳市水环境中新型污染物检测与控制重点实验室,广东深圳518001 [2]深圳大学化学与环境工程学院,广东深圳518060 [3]同济大学环境科学与工程学院,污染控制与资源化研究国家重点实验室,上海200092

出　　处：《中国环境科学》2017年第5期1930-1936,共7页China Environmental Science

基　　金：国家自然科学基金(51378368);深圳市科技研发资金项目(CXZZ20150330151321966)

摘　　要：通过24孔板序批实验对群体效应(QS)信号分子C_8-oxo-HSL对铜绿假单胞菌(P.aeruginosa)在污水处理过程中生长特性影响作用进行分析探讨.研究发现C_8-oxo-HSL有效刺激P.aeruginosa大量生长,其对应的阙值浓度为10^(-10)g/L C_8-oxo-HSL.同时推知具有Lux I/R群体效应系统的革兰氏阴性菌可在较低的信号分子浓度作用下大量增殖.C_8-oxo-HSL还可有效刺激P.aeruginosa分泌大量蛋白质至紧密型胞外聚合物(TB-EPS)中,其对应的阙值浓度为10^(-7)g/LC_8-oxo-HSL.但C_8-oxo-HSL对P.aeruginosa的TB-EPS中多糖没有明显影响作用.C_8-oxo-HSL通过促使P.aeruginosa的增殖和团聚从而增强菌胶团稳定性.此外C_8-oxo-HSL可促进P.aeruginosa生物膜形成.但由于P.aeruginosa生物膜的形成同时和细菌菌量与胞外聚合物浓度直接相关.所以P.aeruginosa生物膜快速生长的C_8-oxo-HSL阙值浓度相对较高(约10^(-8)g/L).N-(3-oxo octanoyl)-L-homoserine lactone(C8-oxo-HSL)is a commonly detected quorum sensing molecule in both natural and built environments.Although it is known that Pseudomonas aeruginosa CICC23618can secrete C8-oxo-HSL,behavioral changes of P.aeruginosa under the influence of C8-oxo-HSL remain unelucidated.Due to unique bioactivities often harbored by P.aeruginosa species important to many biological processes(e.g.,activated sludge process),it is fundamentally important to understand effects of C8-oxo-HSL to P.aeruginosa.The study showed that C8-oxo-HSL at as low as10-10g/L level could enhance the growth rate of P.aeruginosa.Thus,it is rational to predict that the growth of gram-negative bacteria bearing the LuxI/R quorum sensing system might also be enhanced by HSL molecules at low concentrations.The study also observed that10-7g/L C8-oxo-HSL was sufficient to cause P.aeruginosa to secrete a relatively high amount of proteins into its tightly bound EPS,but the production of polysaccharide unaffected by C8-oxo-HSL.Biofilm formation is a complex biological process involving combined effects of cell growth and EPS production.Given the effects mentioned above,it is not surprised to see that10-8g/L C8-oxo-HSL eventually enhanced the formation of P.aeruginosa biofilm and improved the stability of its cell aggregates.Overall,the study quantitatively demonstrated how C8-oxo-HSL as a representative of HSL quorum sensing family impacted the physiology of P.aeruginosa and revealed its potential implications in biological processes.

关 键 词：群体效应 铜绿假单胞菌 C8-oxo-HSL 菌胶团 生物膜 

分 类 号：X171[环境科学与工程—环境科学]