RBER生物降解溴酸盐及微生物群落结构分析  

Removal of bromate by a rotating biofilm-electrode reactor(RBER) and microbial community analyses

作　　者：钟宇[1,2] 杨麒[3] 李小明[3] 刘湛[1,2] 向仁军[1,2] 文涛[1,2] 成应向[1,2] 尤翔宇[1] 罗达通 ZHONG Yu;YANG Qi;LI Xiao-ming;LIU Zhan;XIANG Ren-jun;WEN Tao;CHENG Ying-xiang;YOU Xiang-yu;LUO Da-tong(Hunan Research Academy of Environment Science, Changsha 410004, China;Hunan Key Laboratory of Water Pollution Control Technology, Changsha 410004, China;College of Environmental Science and Engineering,Hunan University, Changsha 410082, China)

机构地区：[1]湖南省环境保护科学研究院,湖南长沙410004 [2]水污染控制技术湖南省重点实验室,湖南长沙410004 [3]湖南大学环境科学与工程学院,湖南长沙410082

出　　处：《中国环境科学》2017年第5期1945-1953,共9页China Environmental Science

基　　金：国家自然科学基金资助项目(51378188;51478170);国家国际科技合作专项项目(2013DFG91190)

摘　　要：溴酸盐(BrO_3^-)是由于含溴离子(Br^-)的水体经过臭氧或加氯消毒后产生的消毒副产物,在毒理致癌性分级上被国际癌症研究机构定为II类致癌物.实验设计了一种旋转电极生物膜反应器系统(RBER),可将浓度范围为150~800μg/L的溴酸盐降解,且溴酸盐被完全还原为溴离子,没有其他中间产物生成.在反应器运行过程中发现,硝酸盐与溴酸盐对氢电子供体存在明显的竞争关系,且硝酸盐对氢电子供体竞争能力强于溴酸盐.选取反应器运行不同阶段的生物膜样品进行DNA提取和高通量测序分析微生物群落多样性.结果表明,优势菌属主要有Bacillus(枯草杆菌属)、Pseudomonas(假单胞菌属)和Lactococcus(乳球菌属)3类,其在总菌属中相对丰度占比分别为37.0%、16.2%和11.1%,可能是实现溴酸盐生物降解的主要优势菌群.Bromate is an oxyhalid disinfection byproduct(DBP)during chlorination or ozonation of bromide-containing water,which has been classified as a group II carcinogen(as a possible human carcinogen)by the International Agency for Research on Cancer(IARC).In this study,an auto-hydrogenotrophic rotating biofilm-electrode reactor(RBER)was designed for bromate removal,and the running tests confirmed that the RBER system could completely reduce150~800μg/L bromate to bromide without accumulation of by-products.The competition for the electron donor H2appeared between bromate and nitrate during the RBER operation,and H2-utilization of NO3-was prior to BrO3-when H2was limiting.The high-throughput sequencing was employed to investigate the microbial communities of six biofilm samples from different stage of RBER.According to16S rRNA gene sequencing,the dominant genus-level bacteria were Bacillus(37.0%),Pseudomonas(16.2%)and Lactococcus(11.1%).These three species are dominant genus-level bacteria,which may be the main bromate-reducing bacteria in RBER.

关 键 词：溴酸盐 硝酸盐 高通量测序 微生物群落 

分 类 号：X172[环境科学与工程—环境科学]