载顺铂纳米脂质体对荷宫颈癌HeLa细胞移植瘤裸鼠的体内作用  

Effects of cisplatin-alginate conjugate liposomes in cervical cancer-bearing nude mice

作　　者：高华[1] 王云飞[2] 王文静[1] 张梅莹[1] 李青[1] 丁莹[1] 狄文[1] GAO Hua;WANG Yun-fei;WANG Wen-jing;ZHANG Mei-ying;LI Qing;DING Ying;DI Wen(Department of Obstetrics and Gynecology, Shanghai Key Laboratory of Gynecologic Oncology, Renji Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200127, China;Department of Gynecology, Affiliated Hospital of Jining Medical University, Jining 272029, China)

机构地区：[1]上海交通大学医学院附属仁济医院妇产科,上海市妇科肿瘤重点实验室,上海200127 [2]济宁医学院附属医院妇科,济宁272029

出　　处：《上海交通大学学报（医学版）》2017年第5期606-610,共5页Journal of Shanghai Jiao tong University:Medical Science

基　　金：上海市卫生和计划生育委员会项目(20134205)~~

摘　　要：目的·建立荷宫颈癌Hela细胞移植瘤裸鼠模型,检测载顺铂纳米脂质体对该模型小鼠的体内作用和安全性。方法·构建Hela细胞皮下成瘤裸鼠模型。观察载顺铂纳米脂质体作用后荷瘤裸鼠的一般状态、肿瘤生长情况,测定及比较瘤体大小和质量,称量并比较裸鼠体质量,对表皮生长因子受体(EGFR)在瘤体中的表达进行免疫组化检测。结果·(1)Hela细胞皮下成瘤裸鼠的肿瘤组织中EGFR高表达。(2)载顺铂纳米脂质体显著抑制荷瘤小鼠皮下肿瘤的生长(P=0.000)。(3)载顺铂纳米脂质体组(CS-EGF-Lip)的肿瘤体积抑瘤率达80.22%,瘤体质量抑制率达58.60%,抑瘤效果明显优于顺铂组(P=0.000)。(4)载顺铂纳米脂质体对荷瘤小鼠体质量的影响最小(P=0.000)。结论·载顺铂纳米脂质体给药系统较顺铂的抑瘤作用显著,可以更有效、安全地发挥抑制实体瘤瘤体生长的作用。Objective·To establish human cervical cancer xenografts in nude mice and investigate the antitumor therapeutic effects and safety of EGF modified cisplatin-alginate conjugate liposomes.Methods·Cervical cancer-bearing mouse model was established by subcutaneously injecting Hela cells in nude mice.General state and xenograft growth of the mice were observed.Tumor volumes,tumor weights,and the body weights of mice during the treatment were recorded and analyzed.The expression levels of EGFR in xenografts were detected by immunohistochemistry.Results·①EGFR was highly expressed in the xenografts.②CS-EGF-Lip inhibited the tumor growth effectively(P=0.000).③The inhibition rates of tumor volume and tumor weight of CS-EGF-Lip were80.22%and58.60%respectively,which were better than those of cisplatin(P=0.000).④CS-EGF-Lip had minimal influence on body weight in mice(P=0.000).Conclusion·CS-EGF-Lip has more effective antitumor effects than cisplatin in cervical cancer-bearing mice,which can inhibit tumor growth of solid tumors with enhanced efficacy and safety.

关 键 词：纳米粒 宫颈癌 肿瘤生长 

分 类 号：R737.33[医药卫生—肿瘤]