Porous metal-organic framework as sorbent and LC-MS/MS as detector to identify aflatoxins in peanut  

作　　者：Huali Xie Qi Zhang Peiwu Li Xiupin Wang Na Wei Li Yu Liangxiao Zhang Wen Zhang 

机构地区：[1]Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan, China [2]Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Wuhan, China [3]Key laboratory of Detection for Aflatoxins, Ministry of Agriculture, Wuhan, China [4]Laboratory of Risk Assessment for Oilseeds Products (Wuhan), Ministry of Agriculture, Wuhan, China [5]Quality Inspection and Test Center for Oilseeds Products, Ministry of Agriculture, Wuhan, China [6]Institutions of Agricultural Product Quality Standrad and Testing Research, Tibet Academy of Agricultural and Animal Husbandry Sciences, Lhasa, Tibet, China

出　　处：《Oil Crop Science》2017年第2期95-105,共11页中国油料作物学报(英文版）

摘　　要：Aflatoxins are the most potent spontaneous carcinogen known as secondary metabolite. Aflatoxins detection in peanut is challenging on account of inherent complexity of high fatty matrices. A modified Quick Easy Cheap Effective Rugged Safe (QuECh-ERS) method couple with liquid chromatograph-mass spectrometer (LC-MS/MS) has been developed to monitor aflatoxins in peanut. It?s a simple and rapid sample preparation for aflatoxins analysis compared with common method. Microporous metal-organic framework MIL-101 (Cr) was used as absorbent in this research. Promising properties of high porosity and surface area endowed MIL-101 (Cr) with efficiently aflatoxin enrichment with other matrix exclusion from high fatty sample. Negligible matrix effect was observed due to efficient extract clean up with MIL-101 sorbent. Recoveries ranged from 73.5% to 98.4% for aflatoxins, with relative standard deviations less than 7%. Limit of detection (LOD) and limit of quantitation (LOQ) for aflatoxins in peanut were 0.05-0.1 μg/kg and 0.2-0.6 μg/kg respectively.By using method described in this research, 15 real samples were successfully analyzed in which 6 samples were contaminated. MIL-101(Cr) was used to reduce matrix effect and develop a quick and effective method for aflatoxins detection in peanut via LC-MS/MS for the first time.

关 键 词：QuEChERS method METAL-ORGANIC framework PEANUT AFLATOXINS LC-MS/MS 

分 类 号：S435.652[农业科学—农业昆虫与害虫防治]