长链非编码胰RNA ROR通过notch1蛋白调控腺癌细胞的增殖凋亡  

Effect of long non-coding RNA ROR on proliferation and apoptosis of pancreatic cancer cells by regulating notch1 protein

作　　者：李冰[1] 熊正方[1] 郭亚民[1] LI Bing;XIONG Zhengfang;GUO Yamin(Department of General Surgery,Qinghai Provincial People′s Hospital,Xining 810000,China)

机构地区：[1]青海省人民医院,西宁市810000

出　　处：《实用医学杂志》2017年第12期1922-1927,共6页The Journal of Practical Medicine

摘　　要：目的:研究长链非编码RNA ROR(lncRNA ROR)对胰腺癌细胞增殖凋亡的影响,并探讨其机制。方法:以胰腺癌细胞BxPC-3为研究对象,RT-PCR检测ROR与notch1的RNA水平,Western blot检测notch1蛋白的变化。通过RNAhybird及萤光素酶报告实验,探索ROR对notch1的调控机制;采用CCK-8、TUNEL检测细胞增殖凋亡的变化。结果:ROR与notch1在胰腺癌组织中均高表达,两者存在正相关性。BxPC-3细胞过表达ROR后,细胞增殖活性升高,DNA损伤阳性细胞比例降低,伴随notch1的mRNA及蛋白水平升高。萤光素酶报告实验显示:ROR可与notch1的调控因子miR-137结合并抑制其活性。将ROR与si-notch1共转染BxPC-3细胞,与对照组相比,pcDNA-ROR+si-notch1组细胞增殖活性降低,TUNEL阳性细胞比例增加,差异均具有统计学意义。结论:lncRNA ROR通过促进notch1蛋白表达,调控胰腺癌细胞的增殖凋亡。Objective To explore the mechanism of long chain noncoding RNA RORin regulating prolifer?ation and apoptosis of pancreatic cancer cell.Methods Pancreatic cancer cell line BxPC?3was selected.TheRNA level of lncRNA ROR and notch1was detected by RT?PCR.Notch1protein level was detected by Westernblot.The regulating relationship between lncRNA ROR and notch1was analyzedby RNAhybird and luciferase re?porter assay.At last,CCK?8and TUNEL were applied to detectthe proliferation and apoptosis of cell line.Re?sults lncRNA ROR and notch1were highly expressed in pancreatic cancer tissue,compared with normal tis?sues.There was positive correlation between them.lncRNA ROR was over?expressed in BxPC?3,cell proliferationactivity was increased and the percentagesof DNA damaged positive cells was decreased,accompanied by in?creased levels of notch1mRNA and protein.Luciferase assay confirmed that ROR could bind to notch1andinhibit its activity by miR?137.Compared with control group,the proliferation of pcDNA?ROR+si?notch1cellsreduced and the proportion of TUNEL positive cells increased.The differences were statistically significant.Conclusionl ncRNA ROR regulated the proliferation and apoptosis of pancreatic cancer cells by promoting theexpression of notch1.

关 键 词：胰腺癌 长链非编码RNA NOTCH1蛋白 凋亡 

分 类 号：R735.9[医药卫生—肿瘤]