超级早稻中早39稻瘟病抗性的系谱分析  

Genealogical Analysis on Resistance to Magnaporthe oryzae of Super Early Rice Zhongzao 39

作　　者：梁燕[1] 季芝娟[1] 曾宇翔[1] 张翠真[2] 吴晗霖 杨长登[1] LIANG Yan;JI Zhijuan;ZENG Yuxiang;ZHANG Cuizhen;WU Hanlin;YANG Changdeng(State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 310006, China;College of Life Science, Yangtze University,Jingzhou 434023, China)

机构地区：[1]中国水稻研究所/水稻生物学国家重点实验室,杭州310006 [2]长江大学生命科学学院,湖北荆州434023

出　　处：《中国水稻科学》2017年第4期441-446,共6页Chinese Journal of Rice Science

基　　金：中央级公益性科研院所基本科研业务费资助项目(2014RG001-3)

摘　　要：【目的】研究常规超级早籼稻品种中早39持久高抗稻瘟病的遗传基础,【方法】利用田间抗性鉴定、稻瘟病抗性基因分子标记检测和基因测序的方法,分析中早39稻瘟病持久抗性的遗传基础。【结果】中早39、金早47和中组3号对7个稻瘟病抗性基因Piz、Pi9、Pib、Pi36、Pi64、Pi-d2和Pi-d3的分子标记均呈阳性条带;且中早39与金早47、中组3号对5个稻瘟病生理小种的抗性表现完全相同。中早39与金早47、中组3号无论是表型还是基因型都完全一致;序列分析结果表明中早39系谱中的供试材料均含有Pi-d2抗病等位基因。【结论】中早39的抗稻瘟病特性经中组3号,来源于金早47。含有Pi-d2的地谷与中早39、金早47和中组3号对5个稻瘟病生理小种的表型相同。【Objective】To clarify the genetic basis of the durable resistance to Magnaporthe oryzae of the super early rice Zhongzao39,【Method】blast resistance assessment,molecular marker screening and gene sequencing were conducted.【Result】Zhongzao39,Jinzao47and Zhongzu3showed positive bands to all the molecular markers for7resistant genes Piz,Pi9,Pib,Pi36,Pi64,Pi-d2and Pi-d3;The resistance performance of Zhongzao39,were fully consistent with Jinzao47and Zhongzu3to the5isolates collected from Zhejiang and Jiangxi Province,China;Molecular sequencing results showed that all the test materials contain Pi-d2resistant alleles.【Conclusion】These results showed that the genetic basis of durable resistance to Magnaporthe oryzae of Zhongzao39was contributed by Jinzao47and Zhongzu3.

关 键 词：分子标记 抗性基因 基因测序 系谱分析 

分 类 号：S435.111.41[农业科学—农业昆虫与害虫防治] S511.034[农业科学—植物保护]