TRAF6在粪肠球菌感染人成骨样细胞炎症反应中的作用  

Role of TRAF6 in inflammatory responses of human osteoblast-like cells with Enterococcus faecalis

作　　者：卢煜[1] 刘成霞[1] 刘忠俊[1] LU Yu;LIU Chengxia;LIU Zhongjun(Department of Endodontics, Stomatological Hospital, Southern Medical University, Guangzhou 510280, China)

机构地区：[1]南方医科大学口腔医院牙体牙髓科,广东广州510280

出　　处：《口腔疾病防治》2017年第7期420-425,共6页Journal of Prevention and Treatment for Stomatological Diseases

基　　金：广东省医学科学技术研究基金(B2014028)

摘　　要：目的探讨肿瘤坏死因子受体相关因子6(tumor necrosis factor receptor-associated factor 6,TRAF6)在粪肠球菌引发的人成骨样细胞MG63炎症反应中的作用。方法采用si RNA瞬时干扰沉默MG63细胞的TRAF6基因,用粪肠球菌灭活全菌及其脂磷壁酸(lipoteichoic acid,LTA)刺激MG63细胞不同时间,定量PCR检测细胞中Toll样受体2(toll-like receptor 2,TLR2)和TRAF6的表达量;ELISA法检测MG63细胞产生的致炎因子白细胞介素1β(interleukin 1β,IL-1β)和肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)表达量。结果粪肠球菌及其LTA感染MG63细胞,TLR2受体和TRAF6的基因水平都有不同程度的上升(P<0.05)。IL-1β和TNF-α的表达量都显著增高(P<0.05)。用si RNA抑制MG63细胞TRAF6表达后,促炎因子IL-1β和TNF-α的表达量显著下降(P<0.05)。结论 MG63细胞主要通过TLR2受体来识别粪肠球菌及其毒性成分。粪肠球菌引起的根尖周感染中,其主要毒力因子是其细胞壁上的LTA。Objective Explore the role and status of tumor necrosis factor receptor-associated factor6(TRAF6)inthe inflammatory response of human osteoblast-like cells MG63which was triggered by Enterococcus faecalis(E.faecalis)and its lipoteichoic acid(LTA).Methods SiRNA technology was applied to silence the TRAF6gene of MG63cells,Using E.faecelis and its LTA to stimulate the silence MG63cells with different hours.After that,using real-timePCR technology to detect toll-like receptor2(TLR2)and TRAF6gene expression and using ELISA assay to detect pro-inflammatory cytokines interleukin-1βand TNF-alpha expression levels.Results When MG63cells was infected byE.faecalis,its LTA,TLR2and TRAF6gene level has increased to varying degrees(P<0.05);interleukin-1βand TNF-alpha expression was significantly higher(P<0.05).When TRAF6gene of MG63cells was silenced by siRNA,pro-in-flammatory cytokines interleukin-1β,interleukin-6,interleukin-8and TNF-alpha expression decreased significantly(P<0.05).Conclusion E.faecalis and its toxic components is identified by MG63cells mainly through TLR2receptors.The major virulence factor in periapical infections caused by E.faecalis is LTA.

关 键 词：粪肠球菌 脂磷壁酸 TOLL样受体2 人成骨样细胞 肿瘤坏死因子受体相关因子6 

分 类 号：R781.05[医药卫生—口腔医学]