甲醛复合PM2.5致小鼠血液毒性的研究  

Formaldehyde and PM_(2.5) induced hepatotoxicity in mice

作　　者：閤静 郭晴[1] 江清英 卢美玲[1] 赵云[1] 朱雨晴[1] 杨旭[1] 李睿[1] GE Jing;GUO Qing;JIANG Qing-ying;LU Mei-ling;ZHAO Yun;ZHU Yu-qing;YANG Xu;LI Rui(Hubei Key Laboratory of Genetic Regulation and Integrative Biology, School of Life Sciences, Central China Normal University, Wuhan 430079, China)

机构地区：[1]华中师范大学生命科学学院,遗传调控与整合生物学湖北省重点实验室,湖北武汉430079

出　　处：《中国环境科学》2017年第7期2740-2748,共9页China Environmental Science

基　　金：国家自然科学基金面上资助项目(21577045);国家自然科学基金重点资助项目(51136002);中央高校基本科研业务费探索创新项目(CCNU15A02028)

摘　　要：为探究甲醛(FA)复合PM_(2.5)对小鼠血液毒性的影响及其可能的机制,分别从血液,造血器官(骨髓、脾脏),髓系祖细胞3个层面的损伤进行系统研究.以雄性Balb/c小鼠为实验对象,把小鼠随机分为4组(对照组、PM_(2.5)组、FA组、PM_(2.5)+FA组),对小鼠进行全血细胞计数;观察骨髓、脾脏病理学的变化;检测骨髓、脾脏及髓系祖细胞的氧化损伤(ROS,GSH),DNA损伤(DPC,8-OH-d G),细胞凋亡(caspase-3)指标.结果表明,骨髓、脾脏分别出现不同程度的病理学变化;骨髓、脾脏,髓系祖细胞的氧化损伤,DNA损伤,细胞凋亡水平也有上升趋势.FA复合PM_(2.5)暴露会导致小鼠的血液毒性,氧化应激,及其下游的DNA损伤可能是FA复合PM_(2.5)致小鼠血液毒性的一种重要机制.In order to explore the influence and the underlying mechanism of FA and PM2.5on hepatotoxicity,we conducted systematic research from three aspects,including toxicity of blood,hematopoietic organs(bone marrow,spleen)and myeloid progenitor.Male Blab/c mice were taken as experimental object,and were randomly divided into four groups:the control group;PM2.5-exposure group;FA-exposure group;PM2.5+FA-exposure group.Blood samples were firstly collected from mice caudal vein to conduct the complete blood count.Then the histopathological change of bone marrow and spleen(H&E staining)were observed.Furthermore,the oxidative damage(ROS,GSH),DNA damage(DPC,8-OH-dG)and apoptosis(caspase-3)in bone marrow,spleen and myeloid progenitor were detected.Results showed different degree of pathological changes in the bone marrow,spleen;compared with control group,oxidative damage,DNA damage,cell apoptosis level all demonstrated a rising trend in PM2.5,FA,PM2.5+FA group.In conclusion,an exposure combined formaldehyde and PM2.5could cause hepatotoxicity in mice,with a possible mechanism from oxidative stress as well as its downstream DNA damage.

关 键 词：PM2.5 甲醛 复合暴露 血液毒性 

分 类 号：X174[环境科学与工程—环境科学]