供体来源半成熟树突状细胞诱导大鼠肝移植术后免疫低反应的实验研究  

Induction of low immune response by donor-derived semi-mature dendritic cells in rats after liver transplantation

作　　者：李春满[1] 王伟[2] 张捷[3] 唐波[1] 李刚[1] 董丽英[4] 朱磊[5] 付必莽[1] Chun-man Li;Wei Wang;Jie Zhang;Bo Tang;Gang Li;Li-ying Dong;Lei Zhu;Bi-mang Fu(The Fourth Department of Hepatopancreatobliliary Surgery,the Second Affiliated Hospital of Kunming Medical University, Kunming, Yunnan 650101, China;The First Department of Urinary Surgery,the Second Affiliated Hospital of Kunming Medical University, Kunming, Yunnan 650101, China;Hepatobiliary and Pancreatic Research Institute,the Second Affiliated Hospital of Kunming Medical University, Kunming, Yunnan 650101, China;Department of Thyroid and Breast Surgery,the Second Affiliated Hospital of Kunming Medical University, Kunming, Yunnan 650101, China;The First Department of Hepatopancreatobiliary Surgery, the Second Affiliated Hospital of Kunming Medical University, Kunming, Yunnan 650101, China)

机构地区：[1]昆明医科大学第二附属医院肝胆胰外科四病区,云南昆明650101 [2]昆明医科大学第二附属医院泌尿外科一病区,云南昆明650101 [3]昆明医科大学第二附属医院肝胆胰研究所,云南昆明650101 [4]昆明医科大学第二附属医院甲状腺乳腺外科,云南昆明650101 [5]昆明医科大学第二附属医院肝胆胰外科一病区,云南昆明650101

出　　处：《中国现代医学杂志》2017年第16期18-23,共6页China Journal of Modern Medicine

基　　金：云南省卫生科技计划项目(No:2014NS063);云南省科技计划项目(No:2014FB053)

摘　　要：目的研究供体来源半成熟树突状细胞(smDC)诱导大鼠肝移植术后免疫低反应的效果,初步分析其作用机制。方法该实验在成功完成大鼠树突状细胞(DC)培养和鉴定的基础上,进行大鼠肝移植术前输注供体来源DC、术后使用雷帕霉素灌胃,观察大鼠术后血清白细胞介素10(IL-10)和γ干扰素(IFN-γ)、CD4^+CD25^+Treg的变化,并评估急性免疫排斥病理改变。结果使用雷帕霉素后,可以改变血清细胞因子的分泌水平、促进Lewis→BN大鼠肝移植术后CD4^+CD25^+Treg的增殖,减轻术后免疫排斥反应,从而延长Lewis→BN大鼠肝移植术后生存时间。结合术前输注未成熟树突状细胞(imDC)或smDC,可以增强雷帕霉素的相应作用,进一步诱导大鼠肝移植术后免疫低反应,且smDC的作用强于imDC。结论 smDC可以诱导Lewis→BN大鼠肝移植术后免疫低反应。Objective To study the effect of donor-derived semi-mature dendritic cells(smDC)in inducing lowimmune response of rats after orthotopic liver transplantation and analyze its mechanism.Methods In thisexperiment,based on culture and identification of rat dendritic cells,donor-derived dendritic cells were infusedbefore orthotopic liver transplantation,and Rapamycin was used for gastric lavage after surgery.Then theexpression levels of cytokines(IL-10and IFN-γ)and CD4+CD25+Treg changes were observed,and the pathologicalchanges of acute immunological rejection were evaluated.Results Administration of Rapamycin changed serumcytokine secretion,promoted CD4+CD25+Treg proliferation after liver transplantation in the Lewis→BN rats,alleviated postoperative immune rejection,thus prolonged the survival time after liver transplantation.Preoperativeinfusion of immature dendritic cells(imDC)or smDC could enhance the effect of Rapamycin and further induce thelow immune response after liver transplantation in rats,and the effect of smDC was stronger than that of imDC.Conclusions smDC can induce low immune response after liver transplantation of Lewis→BN rats.

关 键 词：肝移植 半成熟树突状细胞 免疫排斥 调节性T细胞 

分 类 号：R-332[医药卫生]