胃癌中高表达的CBX7对细胞迁移、侵袭的影响  

CBX7 expression in human gastric cancer and its role in cell migration and invasion

作　　者：宗华[1] 秦杰[1] 李红春[1] 朱立元[1] Hua Zong;Jie Qin;Hong-chun Li;Li-yuan Zhu(Department of General Surgery, the Third People's Hospital of Shenzhen,Shenzhen, Guangdong 518000, China)

机构地区：[1]广东省深圳市第三人民医院普外科,广东深圳518000

出　　处：《中国现代医学杂志》2017年第16期40-43,共4页China Journal of Modern Medicine

摘　　要：目的探究人胃癌组织中染色盒同源物7(CBX7)的表达及对胃癌细胞侵袭能力的影响。方法收集2013年1月1日-2015年1月1日间于该院普通外科行手术切除的45例胃癌及对应癌旁组织,免疫组织化学染色观察CBX7蛋白表达,分析胃癌组织中CBX7蛋白表达高低与肿瘤临床特征的关系。通过重组质粒在胃癌SGC-7901细胞中过表达CBX7,经Transwell小室实验确定CBX7过表达对胃癌细胞迁移侵袭的作用。结果 CBX7在胃癌组织中表达较癌旁组织下降(t=3.517,P=0.000),胃癌组织低表达CBX7与肿瘤淋巴结转移(χ~2=5.829,P=0.022)及高TNM分期(Ⅲ+Ⅳ期,χ~2=4.465,P=0.047)相关。过表达CBX7对SGC-7901的迁移(t=42.040,P=0.000)及侵袭(t=40.119,P=0.000)具有显著的削弱作用。结论 CBX7在胃癌组织中表达降低,过表达CBX7能够通过抑制胃癌细胞迁移及侵袭发挥抗肿瘤作用。Objective To study the expression of chromobox homolog7(CBX7)in human gastric carcinoma andits role in cell migration and invasion.Methods Immunohistochemical method was applied to detect the expressionof CBX7in gastric cancer and matched tumor-adjacent tissues of45patients.The correlations between CBX7expression and clinical features were analyzed by Pearson chi-square test.Recombinant CBX7plasmid was used toover-express CBX7in SGC-7901cells.The effect of CBX7on cell migration and invasion was measured byTranswell assay.Results The expression of CBX7was significantly down-regulated in the gastric cancer tissues,andthe low expression of CBX7was associated with lymphatic metastasis and advanced TNM stage(stageⅢandⅣ)(P<0.05).Over-expression of CBX7inhibited the migration and invasion of SGC-7901cells(P<0.05).Conclusions CBX7expression is decreased in gastric cancer tissues.Up-regulation of CBX7expression could playan anti-cancer role through inhibition of cell migration and invasion.

关 键 词：CBX7 胃癌 迁移 侵袭 

分 类 号：R735.2[医药卫生—肿瘤]