高级糖基化终末产物受体下调对高迁移率族蛋白B1表达及移植瘤体积的影响  

Influence of down-regulation of RAGE receptor on HMGB1 expression and the volume of transplanted tumor

作　　者：王新军[1] 杨卓[1] 杨如意[1] 周少龙[1] WANG Xinjun;YANG Zhuo;YANG Ruyi;ZHOU Shaolong(Department of Neurosurgery,the Fifth Affiliated Hospital of Zhengzhou University,Zhengzhou 450052,China)

机构地区：[1]郑州大学第五附属医院神经外科,450052

出　　处：《实用医学杂志》2017年第14期2295-2298,共4页The Journal of Practical Medicine

基　　金：河南省教育厅科学技术研究重点项目(编号:14A320078);郑州市科技发展计划项目(编号:20150390)

摘　　要：目的探究高级糖基化终末产物受体(RAGE)下调对胶质瘤细胞系中高迁移率族蛋白B1(HMGB1)的表达及裸鼠移植瘤体积变化的影响。方法用免疫组织化学法,蛋白印迹观察胶质瘤LN229细胞系(分为对照组和实验组)中HMGB1的表达,对照组用生理盐水处理,实验组用RAGE受体阻断剂FPS-ZM1处理,用同样方法检测上述处理后细胞系中HMGB1蛋白的表达水平,其表达的差异采用两独立样本t检验;选取30只Nu/Nu裸鼠并随机分为2组,分别于裸鼠左侧背部相同部位注射上述2种细胞系,于注射后连续6周,共计6次测量裸鼠背部移植瘤体积大小,体积变化的差异采用重复测量资料的方差分析。结果 HMGB1主要表达于细胞核和细胞质中,与对照组细胞系相比,实验组细胞系中HMGB1蛋白表达水平下降(P<0.05),接种对照组的裸鼠移植瘤体积增长速度明显快于实验组(P<0.05),差异存在统计学意义。结论 HMGB1蛋白可能通过RAGE受体参与胶质瘤的生长与侵袭,RAGE受体阻滞剂FPS-ZM1可以明显地降低HMGB1蛋白的表达,抑制移植瘤体积的增长,有望用于胶质瘤细胞凋亡的研究。Objective To explore the down?regulation of advanced receptor for advanced glycation end products(RAGE)on expression of high mobility group protein B1(HMGB1)in glioma cells line and the volume change of transplanted tumor in nude mice.Methods HMGB1expression in glioma LN229cells line(divided into a control group and a study group)was observed by immunohistochemical assay and Western blot.The control group received normal saline,whereas the study group received RAGE receptor blocking agent FPS?ZM1.Expression of HMGB1protein was detected by the same methods.The difference of the expression was examined byindependent sample t test.30Nu/Nu nude mice were randomly divided into two groups;the above two kinds cell lines were injected into the same area of the left back of nude mice.Six weeks after injection,the volume size was measured six times,and the variance of repeated measurement data was used to analyze the difference of the volume change.Results HMGB1protein was mainly expressed in the cytoplasm and nucleus.As compared with the control group,HMGB1protein expression levels were decreased in the study group(P<0.05),the growth rate of transplanted tumor in nude mice was significantly faster in the control group than in the study group,the difference was statistically significant(P<0.05).Conclusions The growth and invasion of HMGB1protein may be involved in glioma by RAGE receptor.RAGE receptor blocker FPS?ZM1can significantly reduce the expression of HMGB1protein and inhibit the growth of transplanted tumor volume.It is expected to be used for the research on glioma cell apoptosis.

关 键 词：高级糖基化终末产物受体 高迁移率族蛋白B1 胶质瘤 细胞凋亡 

分 类 号：R739.41[医药卫生—肿瘤]