Characterization of Aquabacterium parvum sp. Strain B6 during Nitrate-Dependent Fe(Ⅱ) Oxidation Batch Cultivation with Various Impact Factors  

作　　者：Xiaoxin Zhang Ulrich Szewzyk Fang Ma 

机构地区：[1]State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology,Harbin 150001, China [2]Department of Environmental Microbiology, Technical University of Berlin, Berlin 10587, Germany [3]China National Institute of Standardization, Beijing 100191,China

出　　处：《Transactions of Tianjin University》2017年第4期315-324,共10页天津大学学报（英文版）

基　　金：supported by a doctoral scholarship from the Chinese Scholarship Council (CSC);the National Natural Science Foundation of China (No. 51,478,140)

摘　　要：In order to assess the capacity of Aquabacterium parvum sp. strain B6 for nitrate-dependent Fe(II) oxidation, batch cultivation was conducted, and its ability to oxidize Fe(II) coupled to nitrate reduction in the presence of diverse organic substrates was studied. Meanwhile, the nitrate-removal rate of B6 with various impact factors was further optimized by the response surface methodology (RSM). The results show that strain B6 is capable of utilizing different organic compounds as substrates for nitrate reduction. Compared with yeast extract, B6 showed a greater potential of chemical oxygen demand (COD) degradation and cell proliferation with acetate and glucose mediums, respectively, while citrate was not beneficial for this process due to its low consumption rate. RSM analysis demonstrated that the maximum nitrate-reduction rate of 30.64% could be achieved with an initial pH of 7.4, incubation temperature of 25.0 °C, and carbon source concentration of 266.10 mg/L. © 2017, Tianjin University and Springer-Verlag Berlin Heidelberg.In order to assess the capacity of Aquabacterium parvum sp. strain B6 for nitrate-dependent Fe(Ⅱ) oxidation,batch cultivation was conducted, and its ability to oxidize Fe(Ⅱ) coupled to nitrate reduction in the presence of diverse organic substrates was studied. Meanwhile, the nitrate-removal rate of B6 with various impact factors was further optimized by the response surface methodology(RSM). The results show that strain B6 is capable of utilizing different organic compounds as substrates for nitrate reduction. Compared with yeast extract, B6 showed a greater potential of chemical oxygen demand(COD)degradation and cell proliferation with acetate and glucose mediums, respectively, while citrate was not beneficial for this process due to its low consumption rate. RSM analysis demonstrated that the maximum nitrate-reduction rate of 30.64% could be achieved with an initial pH of 7.4,incubation temperature of 25.0 °C, and carbon source concentration of 266.10 mg/L.

关 键 词：Carbon Cell proliferation Iron compounds NITRATES Optimization OXIDATION 

分 类 号：TQ920.1[轻工技术与工程—发酵工程]