西多福韦对HeLa细胞增殖抑制作用的研究  

Anti-proliferative effect of cidofovir on HeLa cells

作　　者：吴五洲[1] 徐祥[2] 欧琴[2] 李蓓[2] 丁娜娜[3] 李明远[3] 杨靖[2,3] WU Wuzhou;XU Xiang;0U Qin;LI Bei;DING Nana;LI Mingyuan;YANG Jing(Taihe Hospital, Hubei University of Medicine, Shiy an, Hubei 442000 , China;Department of Microbiology Hubei University of Medicine y Shiyan y Hubei 442000 , China;Department of Microbiology, West China School of Preclinical and Forensic Medicine, Sichuan University,Chengdu ,Sichuan 610041, China)

机构地区：[1]十堰市太和医院湖北医药学院附属太和医院骨科,湖北十堰442000 [2]湖北医药学院微生物学教研室,湖北十堰442000 [3]四川大学华西基础医学与法医学院微生物学教研室,四川成都610041

出　　处：《安徽医药》2017年第8期1494-1497,共4页Anhui Medical and Pharmaceutical Journal

基　　金：湖北省教育厅科学研究计划项目(B2016113);十堰市科技局市级科学技术研究与开发专项基金项目(16K70);湖北医药学院研究生启动金资助计划项目(2015QDJZR08);2015年湖北省省级大学生创新创业训练计划项目(201510929024)

摘　　要：目的研究抗病毒药西多福韦(CDV)对HeLa细胞的增殖抑制作用。方法采用MTT法分析CDV和阳性对照药顺铂(DDP)对HeLa细胞增殖活力的影响;利用细胞集落形成实验和Giemsa染色技术,观察CDV和DDP导致HeLa细胞凋亡情况;利用流式细胞仪观察CDV和DDP对细胞凋亡及细胞周期影响。结果 MTT法和细胞集落实验结果显示CDV能明显抑制HeLa细胞的增殖活性;流式细胞仪测定显示CDV和DDP使HeLa细胞阻滞于细胞周期的S期,并诱导HeLa细胞发生凋亡。结论 CDV对HeLa细胞具有明显的增殖抑制作用,并能诱导细胞凋亡,CDV有可能成为宫颈癌治疗的另一策略。Objective To study the inhibition of cidofovir(CDV)on proliferation of HeLa cells.Methods MTT assay was used to analyze the influence of CDV and cisplatin(D D P)on HeLa cell proliferation.Clone formation assay and Giemsa staining were used to observe the situation of cell apoptosis caused by CDV and DDP on HeLa cells.Flow cytometry was used to detect the shape and size of apoptotic cells after PI staining.Results CDV could inhibit the proliferation of HeLa cells.Results of flow cytometry assay showed that CDV induced cells arrested in S-phase and cell apoptosis.Conclusion CDV can inhibit HeLa cells proliferation,regulate cell cycle and trigger apoptosis,which makes it become another potential treatment strategy of cervical cancer.

关 键 词：人乳头瘤病毒 HELA细胞 西多福韦 顺钼 细胞凋亡 

分 类 号：R737.33[医药卫生—肿瘤]