Enhancement of Simultaneous Xylose and Glucose Utilization by Regulating ZWF1 and PGI1 in Saccharomyces Cerevisiae  

作　　者：Gaogang Liu Bingzhi Li Chun Li Yingjin Yuan 

机构地区：[1]Key Laboratory of Systems Bioengineering(Ministry of Education),School of Chemical Engineering and Technology,Tianjin University,Tianjin 300072,China [2]SynBio Research Platform,Collaborative Innovation Center of Chemical Science and Engineering(Tianjin),Tianjin University,Tianjin 300072,China [3]School of Life Science,Beijing Institute of Technology,Beijing 100081,China

出　　处：《Transactions of Tianjin University》2017年第3期201-210,共10页天津大学学报（英文版）

基　　金：supported by the National Basic Research Program of China(‘‘973’’Program,No.2013CB733601);the National High Technology Research and Development Program of China(‘‘863’’Program,No.2012AA02A701);the National Natural Science Foundation of China(No.21390203);the Tianjin Municipal Science and Technology Committee(No.13RCGFSY19800)

摘　　要：Xylose utilization is one of the key issues in lignocellulose bioconversion. Because of glucose repression, in most engineered yeast with heterogeneous xylose metabolic pathway, xylose is not consumed until glucose is completely utilized. Although simultaneous glucose and xylose utilization have been achieved in yeast by RPE1 deletion, we regulated ZWF1 and PGI1 transcription to improve simultaneous xylose and glucose utilization by controlling the metabolic flux from glucose into the PP pathway. Xylose and glucose consumption increased by approximately 80 and 72%, respectively, whereas ZWF1 was overexpressed by multi-copy plasmids with a strong transcriptional promoter. PGI1 expression was knocked down by promoter replacement; the glucose and xylose metabolism increased when PGI1p was replaced by weak promoters, SSA1p and PDA1p. ZWF1 overexpression decreased while PGI1 down-regulation increased the ethanol yield to some extent in the recombinant strains. © 2017, The Author(s).Xylose utilization is one of the key issues in lignocellulose bioconversion.Because of glucose repression,in most engineered yeast with heterogeneous xylose metabolic pathway,xylose is not consumed until glucose is completely utilized.Although simultaneous glucose and xylose utilization have been achieved in yeast by RPE1 deletion,we regulated ZWF1 and PGI1 transcription to improve simultaneous xylose and glucose utilization by controlling the metabolic flux from glucose into the PP pathway.Xylose and glucose consumption increased by approximately 80 and 72%,respectively,whereas ZWF1 was overexpressed by multi-copy plasmids with a strong transcriptional promoter.PGI1 expression was knocked down by promoter replacement; the glucose and xylose metabolism increased when PGI1p was replaced by weak promoters,SSA1p and PDA1p.ZWF1 overexpression decreased while PGI1 down-regulation increased the ethanol yield to some extent in the recombinant strains.

关 键 词：METABOLISM XYLOSE YEAST 

分 类 号：Q93[生物学—微生物学]