生防枯草芽孢杆菌210发酵工艺优化  

Optimization of fermentation technology of biocontrol bacterium Bacillus subtilis 210 by response surface analysis

作　　者：邹高溪 赵春田[1] 裘娟萍[1] ZOU Gaoxi;ZHAO Chuntian;QIU Juanping(College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou 310014, China)

机构地区：[1]浙江工业大学生物工程学院,浙江杭州310014

出　　处：《浙江农业学报》2017年第5期799-805,共7页Acta Agriculturae Zhejiangensis

基　　金：浙江省重中之重学科开放研究基金(20150109)

摘　　要：采用响应面法,优化抗真菌的枯草芽孢杆菌210的发酵工艺,提高芽孢产量。结果显示,影响芽孢产量最主要的3个因素为玉米粉、蛋白胨和KH_2PO_4用量,最佳发酵培养基配方为:玉米粉10.75 g·L^(-1)、黄豆粉9.00 g·L^(-1)、蛋白胨6.97 g·L^(-1)、KH2PO40.66 g·L^(-1)、Na Cl 4.00 g·L^(-1)、MgSO_4·7H2_O 0.60 g·L^(-1)、Ca Cl_23.00 g·L^(-1)、MnSO_4·H_2O 0.60 g·L^(-1)。基于优化配方通过单因素优化试验,确定最佳发酵条件为:培养温度31℃、装液量30 m L·250 mL^(-1)、接种量4%。枯草芽孢杆菌210在上述优化后的条件下培养,芽孢产量达到1.64×10^(10)cfu·mL^(-1),较优化前提高6.5倍。In order to increase the spore yield,the fermentation technology of Bacillus subtilis210with antifungal activitywas optimized.It was shown that the main factors influencing spore production were doses of corn flour,peptone and KH2P04.The optimal fermentation medium was as follows:corn flour10.75g.L-1,soybean meal9.00g.L-1,peptone6.97g.L-1,KH2P040.66g.L-1,NaCl4.00g.L-1,MgS04.7H200.60g.L-1,CaCl23.00g.L-1and MnS04.H200.60g.L-1.Based on the single factor optimization experiment results,the optimal culture conditions were as follows-culture temperature31,fluid volume30mL.250mL-1,inoculum size4%.With the optimal fermentation condition,the spore yield of Bacillus subtilis210reached1.64x1010cfu.mL-1,and was increased by6.5fold as compared with the spore yield before fermentation technology optimization.

关 键 词：枯草芽孢杆菌 发酵工艺 响应面法 

分 类 号：S482.7[农业科学—农药学]