基于双向电泳的应激性抑郁症模型大鼠蛋白质组学研究  

Proteomics study of stress-induced depression in rat model based on the two dimensional electrophoresis

作　　者：衣泰龙 涂悦[1] 张赛[1] 文立 孙洪涛[1] 杨程[1] 程世翔[1] YI Tai-long;TU Yue;ZHANG Sai;WEN Li;SUN Hong-tao;YANG Cheng;CHENG Shi-xiang(Tianjin Key Laboratory of Neurotrauma Repair, Institute of Traumatic Brain Injury and Neuroscience, the Affiliated Hospital of Logistics University of Chinese People’s Armed Police Forces, Tianjin 300162, China;Tianjin Key Laboratory of Exercise Physical & Sport Medicine, Tianjin University of Sport)

机构地区：[1]天津市神经创伤修复重点实验室,武警部队脑创伤与神经疾病研究所,武警后勤学院附属医院脑科中心,300162 [2]天津市运动医学重点实验室

出　　处：《天津医药》2017年第8期825-829,共5页Tianjin Medical Journal

基　　金：全军技术产品研究重大项目(AWS15J001);天津市临床医学研究中心科技重大专项(15ZXLCSY00040);天津市自然科学基金面上项目(17JCYBJC25700)

摘　　要：目的筛选应激性抑郁症(STRID)模型大鼠海马组织中的差异表达蛋白,初步探讨STRID的发病机制。方法将20只成年SD大鼠随机分为对照组和STRID组,每组10只。STRID组给予慢性温和不可预知应激(CUMS)干预(包括禁食水、电刺激足底、颠倒昼夜、冰水游泳、倾斜鼠笼、惊吓刺激、夹尾等方法)连续不重复刺激28d建模,对照组在此期间正常喂养。在STRID模型建立完成后提取2组大鼠海马组织进行蛋白双向电泳,筛选出差异表达蛋白点后进行质谱鉴定和蛋白功能分析。结果 2组共鉴定出34个差异表达蛋白,其中STRID组较对照组表达上调18个、下调16个。差异表达蛋白主要集中于细胞质、线粒体、细胞外泌体和髓鞘中,主要参与代谢信号通路、氧化磷酸化信号通路,以及阿尔茨海默症、帕金森病和亨廷顿症信号通路。结论 STRID大鼠海马组织存在蛋白的异常表达和神经信号传导异常,过度的氧化磷酸化可能是其致病机制之一。Objective To screen altered proteins of hippocampus in the stress-induced depression(STRID)rat model,and explore the potential molecular mechanism.Methods Twenty Sprague-Dawley rats were randomly divided into the control group and STRID group,10rats in each group.Chronic unpredictable mild stress(CUMS)methods including fastingfor solids and liquids,electric foot-shock,reversing day and night,cold water swimming,cage tilt,scare stimulation and tailpinch were conducted on STRID rats with no repeats for28days to make up the depression animal model.The control groupwas normally fed during this period.After the stress stimulation,the hippocampus protein samples were used for twodimensional electrophoresis to screen the differentially expressed protein,and then mass spectrum identification andfunction analyze were conducted.Results Compared with the control group,34proteins were altered in STRID group.Among which,18were up-regulated,and16were down-regulated.The differentially expressed proteins mainly located incytoplasm,mitochondrion,extracellular exosome and myelin sheath.The involved signaling pathways included metabolicpathway,oxidative phosphorylation pathway,and Alzheimer’s disease,Parkinson’s disease and Huntington’s diseasepathways.Conclusion The altered proteins and dysfunction of nerve signaling,and the excess of oxidative phosphorylationin hippocampus of STRID rats may be one of the pathogenesises.

关 键 词：抑郁症 蛋白质组学 电泳 凝胶 双向 质谱分析法 大鼠 Sprague-Dawley 慢性温和不可预知应激 应激性抑郁症 

分 类 号：R749.42[医药卫生—神经病学与精神病学]