石莼对磺胺甲恶唑和红霉素胁迫耐受性及指标表征  

Tolerance and indicator characteristics of Ulva pertusa under sulfamethoxazole and erythromycin stress

作　　者：陈友媛[1] 狄玥莉 卢爽[1] 吴丹 孙萍[1] CHEN You-yuan;DI Yue-li;LU Shuang;WU Dan;SUN Ping(Key Laboratory of Marine Environmental Science and Ecology, Ministry of Education; Shandong Provincial Key Laboratory of Marine Environment and Geological Engineering; College of Environmental Science and Engineering, Ocean University of China, Qingdao 266100, China;Urban Planning Bureau, Qingdao 266000, China)

机构地区：[1]中国海洋大学环境科学与工程学院,海洋环境与生态教育部重点实验室,山东省海洋环境地质工程重点实验室,山东青岛266100 [2]山东省青岛市规划局,山东青岛266100

出　　处：《中国环境科学》2017年第8期3114-3122,共9页China Environmental Science

基　　金：青岛市科技攻关项目(12-4-1-58-HY);青岛市政府采购项目(T-20150205-018)

摘　　要：通过海水培养实验,分析了磺胺甲恶唑(sulfamethoxazole,SMZ)和红霉素(erythromycin,ETM)胁迫下石莼生长、生理指标,探究石莼对SMZ和ETM的耐受性.并通过SMZ和ETM胁迫下石莼生理指标主成分分析,筛选SMZ和ETM胁迫响应表征指标.结果表明,低浓度SMZ(0.50mg/L)促进石莼生长,相对生长速率(RGR)显著升高(P<0.05),而ETM≥0.06mg/L抑制石莼生长.生理指标间接反映石莼对SMZ和ETM的耐受性.较高浓度SMZ(≥2.50mg/L)和ETM(≥0.18mg/L)破坏石莼细胞膜透性,相对电导率显著增加(P<0.05);0.50~1.50mg/L SMZ和0.06~0.18mg/L ETM开始抑制石莼光合作用,叶绿素a(Chla)含量、1,6-二磷酸醛缩酶(FBA)活性显著下降(P<0.05);1.50mg/LSMZ和0.06mg/LETM破坏石莼抗氧化系统平衡,引起过氧化氢(H_2O_2)和丙二醛(MDA)含量显著增加(P<0.05),超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽还原酶(GR)活性增加.综合生长和生理指标表明,SMZ胁迫浓度不超过0.50mg/L对石莼生长状况和生理代谢有促进作用,SMZ≥1.50mg/L,ETM≥0.06mg/L超过石莼耐受范围,对石莼造成毒害,且石莼对SMZ的耐受性优于ETM.主成分分析结果表明,表征SMZ胁迫下石莼细胞膜透性、光合作用、抗氧化系统变化的生理指标分别为相对电导率,FBA和类胡萝卜素(Car),MDA,而表征ETM胁迫下这3个生理过程的指标分别为相对电导率,FBA和Car,MDA、CAT和抗坏血酸过氧化物酶(APX).In order to gain fundamental insights into the tolerance and response characteristics of Ulva pertusa under SMZ(Sulfamethoxazole)and ETM(erythromycin)stress,the seawater culture experiment was conducted and a PCA(principal components analysis)was used to investigate its growth and physiology characteristics.The results showed that the growth of Ulva pertusa was promoted at a low-level concentration of SMZ(0.50mg/L),which was indicated by the significant elevation of the RGR(relative growth rate)(P<0.05).However,the growth of Ulva pertusa was inhibited at ETM≥0.06mg/L.In addition,the physiological parameters indirectly reflected the tolerance of Ulva pertusa under SMZ and ETM stress.The cell membrane permeability of Ulva pertusa was destructed at a relatively high-level concentration of SMZ(≥2.50mg/L)and ETM(≥0.18mg/L),which was supported by the significant increase of the relative conductivity(P<0.05).The photosynthesis of Ulva pertusa began to be inhibitedat0.50~1.50mg/L of SMZ and0.06~0.18mg/L of ETM,indicated by the significant decrease of the Chla(chlorophyll a)content and the FBA(1,6-diphosphate aldolase)activity(P<0.05).Significantly increases of H2O2(hydrogen peroxide)content,MDA(malondialdehyde)content(P<0.05)and SOD(superoxide dismutase),CAT(catalase)and GR(glutathione reductase)activities were observed under1.50mg/L SMZ and0.06mg/L ETM stress,which indicated the destruction of the antioxidant system balance.The results showed that the growth and metabolism of Ulva pertusa could be promoted at SMZ concentration of0.50mg/L,but SMZ≥1.50mg/L and ETM≥0.06mg/L were beyond the tolerance capacity of Ulva pertusa,and Ulva pertusa was less vulnerable to SMZ stress than ETM stress.The PCA results showed that response indicators for cell membrane permeability,photosynthesis and antioxidant system of Ulva pertusa under SMZ stress were the relative electrical conductivity,FBA and Car(carotenoid),MDA,respectively,while under ETM stress were relative conductance,FBA and Car,MDA、CAT and APX(ascorbate peroxid

关 键 词：石莼 磺胺甲恶唑 红霉素 耐受性 指标表征 

分 类 号：X171[环境科学与工程—环境科学]