结肠癌患者ΔNp63和NF-κB的表达及意义  

Expression of ΔNp63 and NF-κB in colon cancer patients and significance

作　　者：樊晓静[1] 史志涛[1] 孙昕[1] Xiao-jing Fan;Zhi-tao Shi;Xin Sun(Department of General Surgery, Xinjiang Uyghur Autonomous Region Hospital of Traditional Chinese Medicine Affiliated to Xinjiang Medical University, Urumqi, Xinjiang 830000, China)

机构地区：[1]新疆医科大学附属自治区中医院普外一科三组,新疆乌鲁木齐830000

出　　处：《中国现代医学杂志》2017年第18期28-31,共4页China Journal of Modern Medicine

摘　　要：目的探讨结肠癌患者N末端截短型p63基因(ΔNp63)和核转录因子κB(NF-κB)的表达及意义。方法采用免疫组织化学法检测结肠癌患者ΔNp63和NF-κB的表达;采用实时荧光定量聚合酶链反应(q RT-PCR)和Western blot检测ΔNp63和NF-κB信使核糖核酸(m RNA)和蛋白的表达;采用半胱氨酸的天冬氨酸蛋白水解酶3(Caspase-3)活性检测试剂盒检测凋亡蛋白Caspase-3表达。结果与对照组比较,结肠癌患者ΔNp63蛋白表达升高35.57%,NF-κB蛋白表达升高58.93%,差异有统计学意义(P<0.05);结肠癌患者ΔNp63和NF-κB m RNA和蛋白表达均升高,差异有统计学意义(P<0.05);结肠癌患者Caspase-3活性升高,差异有统计学意义(P<0.05)。结论结肠癌患者ΔNp63和NF-κB呈过度表达状态;ΔNp63和NF-κB的过表达在结肠癌发生、发展机制中可能起到重要作用。Objective To investigate the expression and effect ofΔNp63and NF-kB in patients with colon cancer.Methods The expressions ofΔNp63and NF-kB in patients with colon cancer were detected by immunohistochemical method.The mRNA and protein expressed byΔNp63and NF-kB were detected by real-time polymerase chain reaction(RT-PCR)and Western blot.The expression of Caspase-3in apoptotic cells was detected by Caspase-3activity assay kit.Results Compared with the control group,the level of protein expressed byΔNp63rose by35.57%and the level of protein expressed by NF-kB rose by58.93%,the difference was statistically significant(P<0.05).The mRNA and protein expressed byΔNp63and NF-kB were obviously up-regulated,the differences were statistically significant(P<0.05).The activity of Caspase-3in the colon cancer group was obviously increased,the difference was statistically significant(P<0.05).Conclusions The expressions ofΔNp63and NF-kB in colon cancer patients are over-expressed,and the over-expressedΔNp63and NF-kB may play important roles in the development and progression of colon cancer.

关 键 词：结肠癌 N末端截短型p63基因 核转录因子KB 免疫组织化学 

分 类 号：R735.35[医药卫生—肿瘤]