HPLC同时测定三果汤水提物中没食子酸、柯里拉京及鞣花酸含量  

Content Determination of Gallic Acid,Corilagin and Ellagic Acid in Aqueous Extract from Sanguo Decoction by HPLC

作　　者：李斌[1] 李鑫[2] 范源[1] LI Bin;LI Xin;FAN Yuan(School of Chinese Medicine, Yunnan University of Chinese Medicine, Kunming 650200, China;Hunan Provincial Key Laboratory of Diagnostics in Chinese Medicine, Hunan University of Chinese Medicine, Changsha 410007, China)

机构地区：[1]云南中医学院中药学院,云南昆明650200 [2]湖南中医药大学中医诊断学湖南省重点实验室,湖南长沙410007

出　　处：《中国中医药信息杂志》2017年第9期76-79,共4页Chinese Journal of Information on Traditional Chinese Medicine

摘　　要：目的建立HPLC同时测定三果汤水提物中没食子酸、柯里拉京及鞣花酸含量的方法。方法采用Agilent ZORBAX SB-C18色谱柱(4.6 mm×250 mm,5μm),流动相为乙腈-0.1%磷酸水溶液,梯度洗脱,检测波长为260 nm,柱温30℃,流速为1.0 mL/min。结果没食子酸、柯里拉京及鞣花酸3种成分有较好的基线分离,且峰形较好,分别在0.174~2.61μg、0.04~0.60μg、0.052~0.78μg范围内线性关系良好;平均加样回收率分别为99.98%、99.81%、100.12%,RSD均小于2.0%。结论本方法快速、准确、重复性好,可为三果汤水提物的质量控制提供依据。Objective To establish an HPLC method for the simultaneous determination of contents of gallic acid,corilagin and ellagic acid in aqueous extract from Sanguo Decoction.Methods The analysis was carried out on an Agilent C18column(4.6mm×250mm,5μm),and the mobile phase was composed of acetonitrile and0.1%phosphoric acid aqueous with gradient elution.The detection wavelength was260nm.The flow rate was1.0mL/min at column temperature of30℃.Results Gallic acid,corilagin and ellagic acid were completely separated and the peak shape was good.It showed a good linearity in the concentration range of0.174-2.61μg,0.04-0.60μg,and0.052-0.78μg,respectively.The average recoveries of gallic acid,corilagin and ellagic acid were99.98%,99.81%and100.12%,respectively.The RSD were less than2.0%.Conclusion This method is rapid,accurate and repeatable,which can provide references for the quality control of Sanguo Decoction.

关 键 词：三果汤水提物 高效液相色谱法 没食子酸 柯里拉京 鞣花酸 

分 类 号：R284.1[医药卫生—中药学]