微拟球藻富油藻株筛选及柱状光生物反应器培养评价研究  

SCREENING OF LIPID-RICH NANNOCHLOROPSIS OCEANICA MUTANTS AND EVALUATION USING A BUBBLE COLUMN PHOTOBIOREACTOR

作　　者：何文栋[1] 朱葆华[1] 冯磊力 常雅青 杨官品[2] 潘克厚[1,3] HE Wen-Dong;ZHU Bao-Hua;FENG Lei-Li;CHANG Ya-Qing;YANG Guan-Pin;PAN Ke-Hou(Key Laboratory of Mariculture (Ocean University of China), Ministry of Education, Qingdao 266003, China;College of Marine Life Science, Ocean University of China, Qingdao 266003, China;Laboratory for Marine Fisheries and Aquaculture, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266003, China)

机构地区：[1]海水养殖教育部重点实验室(中国海洋大学),青岛266003 [2]中国海洋大学海洋生命学院,青岛266003 [3]青岛海洋科学与技术国家实验室,海洋渔业科学与食品产出过程功能实验室,青岛266003

出　　处：《水生生物学报》2017年第5期1112-1117,共6页Acta Hydrobiologica Sinica

基　　金：国家重点基础研究发展计划项目(2011CB200901和2011CB200904);国家自然科学基金项目(31372518);宁波大学“水产”浙江省重中之重学科开放基金(xkzsc1517)资助~~

摘　　要：研究以亲脂性荧光染料BODIPY505/515和流式细胞仪为基础,从多株诱变海洋微拟球藻(Nannochloropsis oceanica)中筛选到4株候选富油藻株(MT-1,2,3,4),并利用柱状光生物反应器对诱变株的产油能力进行了综合评价。结果表明,藻株筛选时最佳BODIPY505/515使用浓度为0.87μg/m L,染色时间为10min;4株诱变株产油性能较野生株有较大提高,其中MT-4油脂积累达到了干重的66%,油脂产率比野生型藻株提高了45%,达到了27.32 mg/(L·d)。4株诱变株的脂肪酸组成合适,其中C16和C18之和占78%以上,且主要以饱和脂肪酸和单不饱和脂肪酸为主;多不饱和脂肪酸只占总脂肪酸的6%—8%,非常适合生物柴油生产。研究提供了一种针对海洋微拟球藻富油藻株快速、有效的筛选方法,并以此为基础筛选得到4株极具生物柴油生产潜力的候选藻株,有望用于规模化生产。After industrialization occurred,fossil fuel reserves decreased drastically,and environmental pollutioncaused by fossil fuel consumption became more serious.Thus,clean and renewable energy alternatives have recentlyattracted increasing amounts of attention.Microalgae have many advantages,such as a high growth rate,suitable fattyacid profiles and high lipid content,which makes them one of the most promising sources for renewable energy production.However,making a profit from biodiesel production based on microalgae is difficult because of the high cost.There are various strategies to reduce the cost of biodiesel production,such as breeding the best microalgae strainsthrough chemical mutagenesis,which is one of the most important methods.Although this method is effective,extraordinarilyheavy follow-up work impedes its promotion and application.Fortunately,flow cytometry and lipophilicfluorescent dyes(BODIPY505/515)provide the opportunity to screen for the best mutants using high throughput.In this study,multiple mutants of Nannochloropsis oceanica were obtained by using chemical mutation(nitrosoguanidine,NTG).We developed a method based on flow cytometry and BODIPY505/515,which can screen microalgalcandidates using high throughput.Our results showed that the optimal concentration and staining time for BODIPY505/515were0.87μg/mL and10min,respectively.Based on this method,four outstanding microalgal strains were obtained.To verify the reliabilityof the screening method,we further investigated the performance of these four strains cultivated in a column photobioreactor.The results showed that lipid productivity of the four mutants was remarkably higher than that of the wildtype,and the highest value was27.32mg/(L·d),demonstrating that this method is effective and feasible.To furtherevaluate the potential of candidates for biodiesel production,we investigated factors such as the fatty acid profiles,chlorophyll a,and PSII quantum efficiency.The results indicated that all of these four mutants possessed great potential

关 键 词：生物柴油 海洋微拟球藻 BODIPY505/515 诱变筛选 柱状光生物反应器 

分 类 号：S968.4[农业科学—水产养殖]