菌株Diaphorobacter polyhydroxybutyrativorans SL-205的反硝化特性  

Denitrification performance of the bacterium Diaphorobacter polyhydroxybutyrativorans SL-205

作　　者：张树松 樊月婷 孙兴滨[1] 仇天雷[2] 高敏[2] 王旭明[2,3] ZHANG Shu-song;FAN Yue-ting;SUN Xing-bin;QIU Tian-lei;GAO Min;WANG Xu-ming(College of Life Science,Northeast Forestry University, Harbin 150040, China;Beijing Agro-Biotechnology Research Center, Beijng Academy of Agriculture and Forestry Sciences, Beijing 100097, China;Key Laboratory of Urban Agriculture (North), Ministry of Agriculture, Beijing 100097, China)

机构地区：[1]东北林业大学生命科学学院,哈尔滨150040 [2]北京市农林科学院北京农业生物技术研究中心,北京100097 [3]农业部都市农业(北方)重点实验室,北京100097

出　　处：《中国环境科学》2017年第9期3532-3539,共8页China Environmental Science

基　　金：国家自然科学基金(21077014);北京市自然科学基金(8152016);北京市农林科学院科技创新能力建设专项(KJCX20140420)

摘　　要：为强化硝酸盐污染水的反硝化脱氮,研究了反硝化新菌株Diaphorobacter polyhydroxybutyrativorans SL-205~T利用不同碳源的缺氧反硝化性能,以及利用固体碳源聚羟基丁酸戊酸共聚酯(PHBV)的好氧反硝化特性.结果表明,在缺氧状态下,菌株SL-205~T分别以乙酸钠、琥珀酸钠和PHBV为唯一碳源时,硝酸盐去除率均达到99%以上;当初始硝态氮浓度为315mg/L时,PHBV的最适投加量为2.0g/L.菌株SL-205~T能利用PHBV进行好氧反硝化,当反应进行到36h时,硝酸盐去除率达到94.54%,平均反硝化速率为8.69mg/(L·h),并且在反应结束时没有亚硝酸盐和氧化亚氮的积累.以上结果为该菌株在废水脱氮处理中的应用奠定了实验基础.In order to enhance nitrogen removal from nitrate-contaminated water,a novel bacterial strain,Diaphorobacterpolyhydroxybutyrativorans SL-205T,was investigated to use different carbon sources for anoxic denitrification.Additionally,the aerobic denitrification performance for strain SL-205T was studied using poly(3-hydroxybutyrate-co-3-hydroxyvalerate)(PHBV)as the sole carbon source.The results showed that the removalefficiencies of nitrate reached up to over99%by strain SL-205T using sodium acetate,sodium succinate and PHBV ascarbon sources under the anoxic conditions.The optimum addition dosage of PHBV was2.0g/L with315mg/L of theinitial nitrate nitrogen.Strain SL-205T had the capability to fully remove as much as97.4%of nitrate nitrogen,and theaverage removal rate for nitrate nitrogen was8.69mg/(L·h)after36h under the aerobic conditions.Meanwhile,both nitriteand nitrous oxide were not accumulated.The results above-mentioned would lay an experimental foundation for nitrogenremoval from wastewater by strain SL-205T.

关 键 词：Diaphorobacter polyhydroxybutyrativorans SL-205T 硝酸盐 碳源 聚羟基丁酸戊酸共聚酯 好氧反硝化 

分 类 号：X172[环境科学与工程—环境科学]