HBV感染者脂肪间充质干细胞体外诱导为肝样细胞  

Adipose-derived mesenchymal stem cells from hepatitis B virus infection patients differentiate into hepatocyte-like cells in vitro

作　　者：王彦会[1] 王志茹[1] 孙岩 赵文静[1] 杨姝媛 赵淑丽 Wang Yan-hui;Wang Zhi-ru;Sun Yan;Zhao Wen-jing;Yang Shu-yuan;Zhao Shu-li(Hepatology Hospital of Jilin Province, Changchun 130062, Jilin Province, China;People’s Hospital of SuLln City, Sulan 132600, Jilin Province, China)

机构地区：[1]吉林省肝胆病医院,吉林省长春市130062 [2]舒兰市人民医院,吉林省舒兰市132600

出　　处：《中国组织工程研究》2017年第25期3984-3989,共6页Chinese Journal of Tissue Engineering Research

摘　　要：背景:有报道显示,人脂肪间充质干细胞在体外特定为环境下可诱导分化为具有白蛋白合成功能及分泌尿素功能的肝样细胞。目的:探讨乙型肝炎病毒感染者皮下脂肪组织间充质干细胞体外向肝样细胞分化的潜能。方法:胶原酶消化乙型肝炎病毒感染者脂肪组织,结合贴壁培养法获得脂肪间充质干细胞。采用三阶段诱导法诱导脂肪间充质干细胞向肝样细胞分化,观察其形态变化,免疫组织化学染色检测肝细胞相关蛋白甲胎蛋白、白蛋白和细胞角蛋白18表达,糖原染色法检测细胞糖原合成功能。结果与结论:脂肪间充质干细胞经3阶段诱导培养后,大部分细胞成多角形,类肝细胞形态;免疫组织化学染色结果显示,诱导培养后脂肪间充质干细胞白蛋白、细胞角蛋白18和甲胎蛋白染色阳性,且细胞白蛋白和细胞角蛋白18的表达随着诱导培养时间的延长逐渐增加;诱导培养后的脂肪间充质干细胞具有糖原合成功能,过碘酸雪夫试剂染色阳性。结果表明,乙型肝炎病毒感染者皮下脂肪组织间充质干细胞经成肝诱导培养后可分化为有功能的肝样细胞。BACKGROUND:It has been reported that human adipose-derived mesenchymal stem cells can be induced to differentiate into hepatocyte-like cells with the function of albumin synthesis and urea secretion in vitro.OBJECTIVE:To investigate the potential of adipose-derived mesenchymal stem cells differentiating into hepatocyte-like cells in vitro.METHODS:Adipose-derived mesenchymal stem cells were isolated from the subcutaneous fat of hepatitis B virus infection patients by collagenase digestion and adherent method.Adipose-derived mesenchymal stem cells were induced by three-phase induction method and observed morphologically.The expression levels of alpha-fetoprotein,albumin and cytokeratin18were detected by immunohistochemical staining and glycogen synthesis function was detected by glycogen staining method.RESULTS AND CONCLUSION:Most of adipose-derived mesenchymal stem cells induced by three-phase induction method were differentiated into hepatocyte-like cells with polygonal morphology.Immunohistochemistry staining results showed that hepatocyte-like cells expressed alpha-fetoprotein,albumin and cytokeratin18,and the expression levels of albumin and cytokeratin18increased with the culture time.The induced cells had the function of glycogen synthesis and were positive for periodic acid Schiff staining.These results showed that the subcutaneous adipose-derived mesenchymal stem cells could be induced into functional hepatocyte-like cells in hepatitis B virus infection patients.

关 键 词：干细胞 脂肪干细胞 间充质干细胞 HBV感染 脂肪 肝样细胞 

分 类 号：R394.2[医药卫生—医学遗传学]