骨髓间充质干细胞的定向分化潜能  

Directional differentiation of bone marrow mesenchymal stem cells

作　　者：李乔乔 吴振强[1] 张丽君[2] Li Qiao-qiao;Wu Zhen-qiang;Zhang Li-jun(School of Biological Science and Engineering, South China University of Technology, Guangzhou 510006, Guangdong Province, China;School of Applied Chemistry and Biotechnology, Shenzhen Polytechnic, Shenzhen 518055, Guangdong Province, China)

机构地区：[1]华南理工大学生物科学与工程学院,广东省广州市510006 [2]深圳职业技术学院应用化学与生物技术学院,广东省深圳市518055

出　　处：《中国组织工程研究》2017年第25期4085-4090,共6页Chinese Journal of Tissue Engineering Research

基　　金：深圳市科技创新委项目(ZYA201106100083A;GGJS20130331152344401);深圳职业技术学院校重点项目(601522k27009)~~

摘　　要：背景:骨髓间充质干细胞因其具有增殖传代能力强、多向分化潜能、不存在伦理问题和排斥反应等特点,是最有前途的组织工程种子细胞。目的:综述各种条件诱导骨髓间充质干细胞定向分化的最新研究进展。方法:由第一作者检索2001年1月至2016年9月中国期刊全文数据库(CNKI)、PubMed数据库及万方数据库相关文章,检索词为"骨髓间充质干细胞、定向分化","BMSCs、Directional differentiation"。检索文献类型为实验研究、综述。检索文献量总计48篇。结果与结论:骨髓间充质干细胞具有多种分化潜能,在体外能被细胞因子、化学药物、中药及其提取物、物理刺激等诱导分化为成骨细胞、软骨细胞、脂肪细胞、心肌细胞和神经细胞等。在体外建立切实可行的诱导分化处理条件,提高骨髓间充质干细胞定向诱导的效率,是骨髓间充质干细胞最终应用于临床治疗的基础工作。BACKGROUND:Bone marrow mesenchymal stem cells is the most promising tissue engineering seed cells because of their strong proliferation and passage ability,multi-directional differentiation potential,as well as no ethical problems and no rejection.OBJECTIVE:To review the recent advances in the directional differentiation of bone marrow mesenchymal stem cells induced by various conditions METHODS:The first author retrieved CNKI,PubMed,Wanfang databases for relevant articles published from January2001to September2016.The keywords were“bone marrow mesenchymal stem cells,BMSCs,directional differentiation”in Chinese and English,respectively.Literature addressing experimental research and review were selected,and finally48articles were included.RESULTS AND CONCLUSION:Bone marrow mesenchymal stem cells can be induced in vitro to differentiate into chondrocytes,osteoblasts,adipocytes,and nerve cells by cytokines,chemical drugs,Chinese herbs and extracts,physical stimulation.To establish feasible induction conditions in vitro and improve cell induction efficiency is the basis for the clinical application of bone marrow mesenchymal stem cells.

关 键 词：干细胞 分化 骨髓间充质干细胞 培养 诱导分化 组织工程 

分 类 号：R318[医药卫生—生物医学工程]