乌腺金丝桃总黄酮的超声提取及其抗氧化活性  

Ultrasonic Extraction of Total Flavonoids from Hypericum attenuatum Choisy and its Antioxidant Activities

作　　者：金杜欣 罗学刚[1] 张克勤[2] 张同存[1,3] JIN Duxin;LUO Xuegang;ZHANG Keqin;ZHANG Tongcun(College of Biotechnology,Tianjin University of Science & Technology,Tianjin 300457,China;School of Animal Sciences,Jilin College of Agricultural Science and Technology,Jilin 132101,China;Institute of Biology and Medicine,Wuhan University of Science and Technology,Wuhan 430000,China)

机构地区：[1]天津科技大学生物工程学院,天津300457 [2]吉林农业科技学院动物科学学院,吉林132101 [3]武汉科技大学生物医学研究院,武汉430000

出　　处：《天津科技大学学报》2017年第5期10-16,共7页Journal of Tianjin University of Science & Technology

基　　金：国家自然科学基金资助项目(31270837);长江学者和创新团队发展计划资助项目(IRT1166)

摘　　要：黄酮类化合物是乌腺金丝桃植物的主要生物活性成分之一.为了研究开发乌腺金丝桃的应用价值,本文以乌腺金丝桃为材料,通过单因素和响应面法得到乌腺金丝桃中总黄酮提取的最佳工艺条件为超声功率500,W、超声温度60℃、乙醇体积分数62%,、液料比37﹕1、提取时间32,min,该条件下乌腺金丝桃中总黄酮提取得率为6.20%,.在10~500,μg/m L范围内,乌腺金丝桃黄酮提取液对不同自由基表现出不同的清除能力,其对DPPH自由基的清除能力相对最强(IC50值为322.58,μg/m L),而对ABTS自由基的清除能力最弱.Flavonoid is one of major bioactive components in Hypericum attenuatum Choisy.In this study,the conditionsfor ultrasonic extraction of total flavonoids from Hypericum attenuatum Choisy were optimized with the methods of singlefactor and response surface to explore the application value of Hypericum attenuatum Choisy.The results showed that theoptimal conditions for the extraction of total flavonoids from Hypericum attenuatum Choisy were500,W ultrasonic power,60℃temperature,62%,ethanol concentration,37﹕1,liquid-solid ratio and32,min extraction time.Under these conditions,the yield of total flavonoids from Hypericum attenuatum Choisy was6.20%,.Its extract exhibited different scavenging capacitieson different radicals,and it has better scavenging capacities on DPPH radical with an IC50,value of322.58,μg/mLand poor scavenging capacities on ABTS radical with the concentration of10-500,μg/mL.

关 键 词：乌腺金丝桃 总黄酮 超声提取 响应面法 抗氧化活性 

分 类 号：R284.3[医药卫生—中药学]