血管新生对局灶性脑缺血再灌注后脑室下区神经干细胞增殖的影响  

Influence of angiogenesis on neural stem cell proliferation in the subventricular zone after focal cerebral ischemia/reperfusion

作　　者：李志宁 韩卫 荣良群 龚爱平 吕尤 单君君 魏秀娥 Li Zhi-ning;Han Wei;Rong Liang-qun;Gong Ai-ping;Lv You;Shan Jun-jun;Wei Xiu-e(Department of Neurology,Second Affiliated Hospital of Xuzhou Medical University, Xuzhou 221006, Jiangsu Province, China;Clinical Laboratory, Second Affiliated Hospital of Xuzhou Medical University, Xuzhou 221006, Jiangsu Province, China)

机构地区：[1]徐州医科大学第二附属医院,神经内科,江苏省徐州市221006 [2]徐州医科大学第二附属医院,检验科,江苏省徐州市221006

出　　处：《中国组织工程研究》2017年第29期4697-4702,共6页Chinese Journal of Tissue Engineering Research

基　　金：“步长杯”江苏省神经病学研究基金课题(局灶性脑缺血后脑室下区细胞结构改变及NMDA受体亚单位蛋白质表达变化,2013年);徐州市科技局课题(NMDA受体诱导血管新生调控缺血后神经干细胞的增殖、分化及机制的研究,编号:KC14SH063)~~

摘　　要：背景:血管新生可能与神经干细胞的增殖有相关性,但仍无统一观点。目的:观察血管新生对局灶性缺血再灌注后大鼠脑室下区神经干细胞增殖的影响。方法:成年雄性SD大鼠,随机分为正常组、假手术组、手术组、侧脑室注射血管内皮生长因子+手术组、侧脑室注射生理盐水+手术组,各组再分为缺血再灌注1,3,7,14 d组,每组12只。线栓法制作局部脑缺血再灌注模型,给予相应干预措施,利用免疫组化法检测各组大鼠脑室下区Nestin的表达,Real time-PCR法检测各组大鼠脑室下区vWF mRNA的表达。结果与结论:①缺血再灌注后脑室下区vWF mRNA、Nestin阳性表达增加;在缺血后7 d,vWF mRNA、Nestin阳性表达均达高峰,提示局灶性缺血再灌注后大鼠脑室下区神经干细胞的增殖与血管新生存在时间相关性;②侧脑室注射血管内皮生长因子+手术组vWF mRNA、Nestin阳性表达较手术组及侧脑室注射生理盐水+手术组明显增高,提示缺血再灌注后血管新生可促进大鼠脑室下区神经干细胞的增殖。BACKGROUND:The angiogenesis may be related to the proliferation of neural stem cells,but there is still no unifiedview.OBJECTIVE:To observe the influence of angiogenesis on neural stem cell proliferation in the subventricular zone of ratsafter focal cerebral ischemia/reperfusion.METHODS:Male Sprague-Dawley rats were randomly divided into normal group,sham group,vascular endothelialgrowth factor(VEGF)+cerebral ischemia/reperfusion group,normal saline(NS)+cerebral ischemia/reperfusion group.The injection was done via the lateral cerebral ventricle.Then,each group was subdivided into four groups(1,2,7,14days after ischemia/reperfusion).Focal cerebral ischemia/reperfusion models were made by the thread method.Aftermodeling,the corresponding intervention was given in each group.The expression changes of Nestin and vWF mRNA inthe subventricular zone were detected in all groups by immunohistochemical staining and real-time PCR,respectively.RESULTS AND CONCLUSION:There was a certain increase in vWF and Nestin positive expression in thesubventricular zone after cerebral ischemia/reperfusion.At7days after ischemia,the expression of vWF mRNA andNestin reached the peak,indicating the proliferation of neural stem cells in the subventricular zone after cerebralischemia/reperfusion is associated with the time of angiogenesis.In addition,the expression of vWF mRNA and Nestinwas significantly higher in the VEGF+cerebral ischemia/reperfusion group than the other two groups,indicatingangiogenesis could promote the proliferation of neural stem cells in the subventricular zone of rats after cerebralischemia/reperfusion.

关 键 词：神经干细胞 脑缺血 血管内皮生长因子类 细胞增殖 组织工程 

分 类 号：R394.2[医药卫生—医学遗传学]