Effects of laccase incubated from white rot fungi on the mechanical properties of fiberboard  

作　　者：Xiaodong Zhu Song Han Yu Liu Guangsheng Chen 

机构地区：[1]Key Laboratory of Bio-based Material Science and Technology (Ministry of Education), Northeast Forestry University, Harbin 150040, People’s Republic of China [2]College of Forestry, Northeast Forestry University,Harbin 150040, People’s Republic of China

出　　处：《Journal of Forestry Research》2017年第6期1282-1289,共8页林业研究（英文版）

基　　金：funded by National Natural Science Foundation of China(31170515);Specialized Research Fund for the Doctoral Program of Higher Education of China(20130062110012);Undergraduate Training Program for Innovation and Entrepreneurship of Northeast Forestry University,China(201410225157)

摘　　要：White rot fungi were optimized to cultivate highly active laccase. The characteristics of laccase incubated by continuous culture were compared with those of direct culture. The enzyme activity of laccase incubated by continuous culture technology reached a higher value on the fifth day of the growth. The optimization incubation time of high activity laccase was the eleventh day. A large amount of highly active laccase can be obtained in a relatively short time by continuous culture to replace traditional laccase. After laccase treatment, the lignin composition of wood fibers were oxidation-catalyzed by laccase. The number of chemical-bonding points between the wood fibers was increased. The wood fibers treated by laccase were fabricated into boards and their mechanical properties improved with the laccase-incubation times. Compared with the fiberboards made from fibers that were pre-treated by laccase of incubation 5 days, the static bending strength of those that were pre-treated by laccase of incubation 11 days was increased by 18.95%, the elastic modulus was increased by 35.49%, and the internal bond strength was increased by 44.11%.White rot fungi were optimized to cultivate highly active laccase. The characteristics of laccase incubated by continuous culture were compared with those of direct culture. The enzyme activity of laccase incubated by continuous culture technology reached a higher value on the fifth day of the growth. The optimization incubation time of high activity laccase was the eleventh day. A large amount of highly active laccase can be obtained in a relatively short time by continuous culture to replace traditional laccase. After laccase treatment, the lignin composition of wood fibers were oxidation-catalyzed by laccase. The number of chemical-bonding points between the wood fibers was increased. The wood fibers treated by laccase were fabricated into boards and their mechanical properties improved with the laccase-incubation times. Compared with the fiberboards made from fibers that were pre-treated by laccase of incubation 5 days, the static bending strength of those that were pre-treated by laccase of incubation 11 days was increased by 18.95%, the elastic modulus was increased by 35.49%, and the internal bond strength was increased by 44.11%.

关 键 词：White rot fungus LACCASE FIBERBOARD Mechanical property 

分 类 号：Q55[生物学—生物化学] TS653.6[轻工技术与工程]