Characterization and Phylogenetic Analysis of Lectin Gene cDNA Isolated from Sea Cucumber (Apostichopus japonicus) Body Wall  

作　　者：XUE Zhuang LI Hui LIU Yang ZHOU Wei SUN Jing WANG Xiuli 

机构地区：[1]College of Fisheries and Life Science, Dalian Ocean University, Dalian 116023, P. R. China [2]Liaoning Province Academy of Analytic Science, Shenyang 110015, P. R. China

出　　处：《Journal of Ocean University of China》2017年第6期1148-1156,共9页中国海洋大学学报（英文版）

基　　金：supported by the National Natural Science Foundation of China (Nos.31502198 and 31572608);The Scientific Research Project of Department of Education of Liaoning Province (No.L2014284);The Dalian Fisheries Association of Industrial Technology Innovation,China (No.201400486);The Doctoral startup fund of Dalian Ocean University (No.HDYJ201407)

摘　　要：As a ‘living fossil' of species origin and ‘rich treasure' of food and nutrition development, sea cucumber has received a lot of attentions from researchers. The cDNA library construction and EST sequencing of blood had been conducted previously in our lab. The bioinformatic analysis provided a gene fragment which is highly homologous with the genes of lectin family, named AjL(Apostichopus japonicus lectin). To characterize and determine the phylogeny of AjL genes in early evolution, we isolated a fulllength cDNA of lectin gene from the body wall of A. japonicus. The open reading frame of this gene contained 489 bp and encoded a 163 amino acids secretory protein being homologous to lectins of mammals and aquatic organisms. The deduced protein included a lectin-like domain. SDS-PAGE analysis showed that AjL migrated as a specific band(about 36.09 kDa under reducing), and agglutinated against rabbit red blood cells. AjL was similar to chain A of CEL-IV in space structure. We predicted that AjL may play the same role of CEL-IV. Our results suggested that more than one lectin gene functioned in sea cucumber and most of other species, which was fused by uncertain sequences during the evolution and encoded different proteins with diverse functions. Our findings provided the insights into the function and characteristics of lectin genes invertebrates. The results will also be helpful for the identification and structural, functional, and evolutionary analyses of lectin genes.As a ‘living fossil’ of species origin and ‘rich treasure’ of food and nutrition development, sea cucumber has received a lot of attentions from researchers. The cDNA library construction and EST sequencing of blood had been conducted previously in our lab. The bioinformatic analysis provided a gene fragment which is highly homologous with the genes of lectin family, named AjL (Apostichopus japonicus lectin). To characterize and determine the phylogeny of AjL genes in early evolution, we isolated a full-length cDNA of lectin gene from the body wall of A. japonicus. The open reading frame of this gene contained 489 bp and encoded a 163 amino acids secretory protein being homologous to lectins of mammals and aquatic organisms. The deduced protein included a lectin-like domain. SDS-PAGE analysis showed that AjL migrated as a specific band (about 36.09 kDa under reducing), and agglutinated against rabbit red blood cells. AjL was similar to chain A of CEL-IV in space structure. We predicted that AjL may play the same role of CEL-IV. Our results suggested that more than one lectin gene functioned in sea cucumber and most of other species, which was fused by uncertain sequences during the evolution and encoded different proteins with diverse functions. Our findings provided the insights into the function and characteristics of lectin genes invertebrates. The results will also be helpful for the identification and structural, functional, and evolutionary analyses of lectin genes.

关 键 词：sea CUCUMBER AGGLUTINATION activity LECTIN space structure 

分 类 号：S917.4[农业科学—水产科学]