High-quality draft genome sequence of Streptomyces agglomeratus 5-1-8 with strong anti-MRSA ability, isolated from the frozen soil of Tibet in China  

作　　者：XiMing Chen Ling Zhang HaiLi Sun ShuYan Li SiJing Chang QingFeng Zhang BingLin Zhang Tuo Chen GuangXiu Liu Paul Dyson 

机构地区：[1]Key Laboratory of Desert and Desertification, Northwest Institute of Eco-Environment and Resources, Chinese Academy of Sciences, Lanzhou, Gansu 730000, China [2]Key Laboratory of Extreme Environmental Microbial Resources and Engineering of Gansu Province, Lanzhou, Gansu 730000, China [3]Xinjiang Institute of Ecology and Geography, Chinese Academy of Science, Urumqi, Xinjiang 830011, China [4]School of Chemistry and Environment Science, Lanzhou City University [5] Lanzhou, Gansu 730000, China [5]College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou, Gansu 730000, China [6]School of Life Sciences, Lanzhou University, Lanzhou, Gansu 730000, China [7]Institute of Life Science, College of Medicine, Swansea University, Singleton Park, Swansea SA2 8PP, UK

出　　处：《Research in Cold and Arid Regions》2017年第5期503-510,共8页寒旱区科学（英文版）

基　　金：supported by the National Natural Science Foundation of China (Grant No. 31400437);the international cooperation program of Gansu (1504WKCA097);the application transformationfoundation of CAS (HHS-CGZH-16-02);UK BBSRC China Partnering Grant (BB/J020419/1)

摘　　要：Streptomyces agglomeratus 5-1-8 with strong anti methicillin-resistant Staphylococcus aureus(MRSA)ability,isolated from the frozen soil of Tibet in China,has a strong ability to kill the multi-drugs-resistant MRSA.To identify the second-ary metabolism ability of this strain,we describe here the phenotypic characteristics of this strain,along with its high-quality draft genome sequence,its annotation,and analysis.The 7.1M draft genome encodes 6,284 putative open reading frames(ORFs),of which 4,416 ORFs were assigned with clusters of orthologous genes(COG)categories.Also,65 t RNA genes and 24 r RNA operons were identified.The genome contains 12 gene clusters involved in antibiotics production and 1 gene cluster involved in anticancer-compounds production;4 gene clusters belong to polyketides and nonribosomal peptides,1gene cluster belong to the butyrolactone,4 gene clusters belong to the bacteriocin or lantipeptide,and 3 gene clusters belong to the others.This genome-sequence data will facilitate efforts to probe the potential of new antibiotics to kill multidrugs-resistant MRSA.Streptomyces agglomeratus 5-1-8 with strong anti methicillin-resistant Staphylococcus aureus(MRSA)ability,isolated from the frozen soil of Tibet in China,has a strong ability to kill the multi-drugs-resistant MRSA.To identify the second-ary metabolism ability of this strain,we describe here the phenotypic characteristics of this strain,along with its high-quality draft genome sequence,its annotation,and analysis.The 7.1M draft genome encodes 6,284 putative open reading frames(ORFs),of which 4,416 ORFs were assigned with clusters of orthologous genes(COG)categories.Also,65 t RNA genes and 24 r RNA operons were identified.The genome contains 12 gene clusters involved in antibiotics production and 1 gene cluster involved in anticancer-compounds production;4 gene clusters belong to polyketides and nonribosomal peptides,1gene cluster belong to the butyrolactone,4 gene clusters belong to the bacteriocin or lantipeptide,and 3 gene clusters belong to the others.This genome-sequence data will facilitate efforts to probe the potential of new antibiotics to kill multidrugs-resistant MRSA.

关 键 词：STREPTOMYCES antibiotic multi-drugs-resistant MRSA genome gene cluster 

分 类 号：R915[医药卫生—微生物与生化药学]