中药臭灵丹有效成分的超临界CO2萃取工艺  

Study on Supercritical CO_2 Extraction Process of Laggera Pterodonta

作　　者：谢维友 于浩飞 张兰春 赵鸿 胡炜彦 王玉涛[3] 王新华[3] 张荣平 XIE Wei-you;YU Hao-fei;ZHANG Lan-chun;ZHAO Hong;HU Wei-yan;WANG Yu-tao;WANG Xin-hua;ZHANG Rong-ping(School of Pharmaceutical Science & Yunnan Key Laboratory of Pharmacology for Natural Products,Kunming Medical University,Kunming Yunnan 650500;Department of Laboratory Animal,Kunming Medical University,Kunming Yunnan 650500;State Key Laboratory of Respiratory Disease,the First Affiliated Hospital of Guangzhou Medical University,Guangzhou Guangdong 510230;Biomedical Engineering Research Center,Kunming Medical University,Kunming Yunnan 650500,China)

机构地区：[1]昆明医科大学药学院暨云南省天然药物药理重点实验室,云南昆明650500 [2]昆明医科大学实验动物学部,云南昆明650500 [3]广州医科大学附属第一医院呼吸疾病国家重点实验室,广东广州510230 [4]昆明医科大学生物医学工程中心,云南昆明650500

出　　处：《昆明医科大学学报》2017年第5期5-9,共5页Journal of Kunming Medical University

基　　金：国家自然科学基金资助项目(81460593);云南省科技重点基金资助项目(2014FA031);云南省科技厅-昆明医科大学联合专项基金资助项目(2015FB003)

摘　　要：目的研究超临界CO_2萃取臭灵丹有效成分的工艺.方法以臭灵丹二醇含量为指标,在单因素试验的基础上,采用正交试验考察超临界CO_2萃取时间、压力、温度对臭灵丹二醇萃取的影响.结果最佳萃取工艺条件为萃取压力25 MPa、时间3 h、温度为50℃.结论在最佳萃取条件下,臭灵丹超临界萃取物得率为4%,臭灵丹二醇含量达12%.表明优选的臭灵丹二醇萃取方法稳定,重复性好.Objective To optimize the supercritical CO2extraction technology of Laggera pterodonta.Methods With the extraction content of pterodondiol as quantitative indicator,the best extraction method wasidentified by single factor experiments.And the effects of extraction pressure,extraction time and extractiontemperature on extraction rate of pterodondiol were investigated by orthogonal tests.Results The optimum extractiontechnology was as follows:extraction pressure25MPa,extraction time3h,and extraction temperature50℃.Conclusions Under these conditions,the average extraction rate was up to4%and the average extraction contentof pterodondiol was up to12%.The optimal extraction process for pterodondiol from Laggera pterodonta isreproducible and stable.

关 键 词：臭灵丹 有效成分 超临界萃取 单因素 正交试验 

分 类 号：R914.1[医药卫生—药物化学]