MAOA μ-VNTR多态性荧光标记自动分型方法的建立及应用  

Establishment and Application An Automatic Typing Method for MAOA μ-VNTR Polymorphism Test using Fluorescence Labeling Techniques

作　　者：姚金勇 胡利平 聂爱婷 黄仁武 邱建波 张秀峰 饶旼 聂胜洁 YAO Jin-yong;HU Li-ping;NIE Ai-ting;HAUNG Ren-wu;QIU Jian-bo;ZHANG Xiu-feng;RAO Min;NIE Sheng-jie(Honghe Prefecture Public Security Bureau,Mengzi 661100;School of Forensic Medicine,Kunming Medical University,Kunming Yunnan 650500;Judicial Identification Center of Kunming Medical University,Kunming Yunnan 650500;NanjianPublic Security Bureau,DaliYunnan 675700 China)

机构地区：[1]红河州公安局,云南蒙自661100 [2]昆明医科大学法医学院 [3]昆明医科大学司法鉴定中心,云南昆明650500 [4]南涧县公安局,云南大理675700

出　　处：《昆明医科大学学报》2017年第6期52-55,共4页Journal of Kunming Medical University

基　　金：国家自然科学基金资助项目(31660310);云南省教育厅科学研究基金资助项目(2015J052)

摘　　要：目的应用毛细管电泳和荧光标记技术,建立MAOA μ-VNTR多态荧光标记自动分型的方法.方法使用Chelex-100法提取DNA后,采用荧光标记和毛细管电泳检测技术对720份云南汉族无关个体血样进行MAOA μ-VNTR基因分型.结果荧光毛细管电泳分型方法能准确、高效地对MAOA μ-VNTR多态性进行分型检测.结论 MAOA μ-VNTR多态性荧光标记自动分型方法操作简便,在针对MAOA μ-VNTR多态性的相关研究中具有良好的应用前景.Objective To establish an automatic typing method for MAOAμ-VNTR polymorphism test using capillary electrophoresis and fluorescence labeling techniques.Methods DNAwas extracted by Chelex-100method.Blood samples from720unrelated Chinese Han individuals in Yunnan were genotyped by capillary electrophoresis and fluorescence labeled primer.Results MAOAμ-VNTR polymorphism fluorescent labeling automatic typing method had high efficiency.And the results from this method were accurate and reliable.Conclusions The operation of MAOAμ-VNTR polymorphism fluorescent labeling automatic typing method is simple.It has good prospects in the research for the MAOAμ-VNTR polymorphismtest.

关 键 词：MAOAμ-VNTR 多态性 荧光标记引物 自动分型 

分 类 号：R394[医药卫生—医学遗传学]