Corilagin对OX-LDL损伤的巨噬细胞中CRP表达的影响  

Effects of Corilagin on Expression of CRP in OX-LDL Injured Macrophage Cells

作　　者：张小超 沈志强 杨仁华 唐传劲 白林英 何波 陈鹏 姚荣成 ZHANG Xiao-chao;SHEN Zhi-qiang;YANG Ren-hua;TANG Chuan-jin;BAI Lin-ying;HE Bo;CHEN Peng;YAO Rong-cheng(School of Pharmaceutical Science & Key Laboratory of Pharmacology for Natural Products of Yunnan Province,Kunming Medical University,Kunming Yunnan 650500;Dept. of Pharmacy,The first People’s Hospital,Qujing Yunnan 655000,China)

机构地区：[1]昆明医科大学药学院暨云南省天然药物药理重点实验室,云南昆明650500 [2]曲靖市第一人民医院药剂科,云南曲靖655000

出　　处：《昆明医科大学学报》2017年第8期1-5,共5页Journal of Kunming Medical University

基　　金：国家自然科学基金资助项目(81260493;81160401;81660613);云南省社会发展计划重点基金资助项目(2015FA021;2012FA002);云南省科技厅-昆明医科大学应用基础研究联合专项基金资助项目(2013FB103;2012FB024);云南省教育厅科学研究基金资助项目(2015Y154)

摘　　要：目的研究Corilagin对氧化型低密度脂蛋白(oxidized low density lipoprotein,OX-LDL)损伤的巨噬细胞中C反应蛋白(C-reactive protein,CRP)蛋白和m RNA表达的影响.方法体外培养小鼠单核细胞源性巨噬细胞,复制OX-LDL诱导损伤模型;Western Blot和RT-PCR方法分别观察Corilagin对巨噬细胞中CRP蛋白和m RNA表达的影响.结果与正常对照组比较,OX-LDL损伤模型组的CRP/β-actin和2-△△Ct值明显增加(P<0.05);与模型组比较,Corilagin 3.125~50μmol/L组的CRP/β-actin和2-△△Ct值明显减少(P<0.05).结论 Corilagin能明显下调OX-LDL损伤的巨噬细胞中CRP蛋白及m RNA的表达.Objective To investigate the effects of Corilagin on expression of C-reactive protein(CRP)inoxidized low density lipoprotein(OX-LDL)injured macrophage cells.Methods In vitro,micemonocyte-derived macrophages were prepared to duplicate the injury model caused by OX-LDL;Western blottingessay was used to determine the effect of Corilagin on CRP protein expression and Real time PCR assay was used tostudy the CRP mRNA expression,respectively.Results Compared with normal control group,the CRP/β-actinand2-ΔΔCt values of OX-LDL injured model group were markedly increased(P<0.05);compared with the modelgroup,Corilagin at3.125-50μmol/L obviously decreased the values of CRP/β-actin and2-ΔΔCt(P<0.05).Conclusion Corilagin could significantly down-regulate protein and mRNA expression of CRP in OX-LDL injuredmacrophage cells.

关 键 词：CORILAGIN 动脉粥样硬化 巨噬细胞 C反应蛋白 

分 类 号：R973[医药卫生—药品]