Fed-Batch Fermentation for Spinosad Production in an Improved Reactor  

作　　者：Chunzhe Lu Jing Yin Chuanbo Zhang Wenyu Lu 

机构地区：[1]School of Chemical Engineering and Technology,Tianjin University,Tianjin 300072,China [2]Key Laboratory of System Bioengineering,Ministry of Education,Tianjin 300072,China [3]SynBio Research Platform,Collaborative Innovation Center of Chemical Science and Engineering,Tianjin 300072,China

出　　处：《Transactions of Tianjin University》2017年第6期530-537,共8页天津大学学报（英文版）

基　　金：supported by the National Natural Science Foundation of China(No.21076148 and No.31270087);Plan of Tianjin Science and Technology Support(11ZCKFSY0100)

摘　　要：As a kind of aerobic bacteria, Saccharopolyspora spinosa exhibits a high demand for oxygen. In the fermentation process, the methods of increasing ventilation and improving agitation speed are usually adopted to achieve higher values of dissolved oxygen. These methods decrease the efficiency of spinosad biosynthesis. In this study, an improved reactor was designed to solve these problems. The exhaust gas reflux device, impellers, and baffles were improved. Furthermore, we established the kinetic models for the cell growth, substrate consumption and spinosad generation in batch fermentation process. The simulation results were in good agreement with the experimental data. Spinosad production reached 583.86 mg/L after employing the suitable feeding strategy by fed-batch fermentation in the improved reactor, whereas it was only 157.01 mg/L before optimization. The method described can provide insight to strengthen spinosad production and can be extended to the culturing process of filamentous aerobic bacteria. © 2017 Tianjin University and Springer-Verlag Berlin HeidelbergAs a kind of aerobic bacteria, Saccharopolyspora spinosa exhibits a high demand for oxygen. In the fermentation process, the methods of increasing ventilation and improving agitation speed are usually adopted to achieve higher values of dissolved oxygen. These methods decrease the efficiency of spinosad biosynthesis.In this study, an improved reactor was designed to solve these problems. The exhaust gas reflux device, impellers,and baffles were improved. Furthermore, we established the kinetic models for the cell growth, substrate consumption and spinosad generation in batch fermentation process. The simulation results were in good agreement with the experimental data. Spinosad production reached 583.86 mg/L after employing the suitable feeding strategy by fed-batch fermentation in the improved reactor, whereas it was only 157.01 mg/L before optimization. The method described can provide insight to strengthen spinosad production and can be extended to the culturing process of filamentous aerobic bacteria.

关 键 词：Aerobic bacteria BACTERIA BIOCHEMISTRY Bioreactors Dissolved oxygen Process control 

分 类 号：TQ920.6[轻工技术与工程—发酵工程]